{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T11:27:30Z","timestamp":1761823650900,"version":"3.33.0"},"reference-count":33,"publisher":"International Union of Crystallography (IUCr)","issue":"6","license":[{"start":{"date-parts":[[2014,5,24]],"date-time":"2014-05-24T00:00:00Z","timestamp":1400889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,5,24]],"date-time":"2014-05-24T00:00:00Z","timestamp":1400889600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>Schistosomiasis is an inflammatory chronic disease that represents a major health problem in tropical and subtropical countries. The drug of choice for treatment, praziquantel, is effective in killing adult worms but fails to kill immature forms and prevent reinfection. One prominent antigen candidate for an anti-schistosomiasis vaccine is the protein Sm21.7 (184 amino-acid residues) from<jats:italic>Schistosoma mansoni<\/jats:italic>, a tegumental protein capable of reducing the worm burden in a murine immunization model. In the present work, the Sm21.7 gene was cloned and expressed in<jats:italic>Escherichia coli<\/jats:italic>and the full-length protein was purified to homogeneity. Crystals of recombinant Sm21.7 suitable for X-ray diffraction were obtained using PEG monomethyl ether 2000 as a precipitant. X-ray diffraction images of a native crystal (at 2.05\u2005\u00c5 resolution) and a quick-cryosoaked NaI derivative (at 1.95\u2005\u00c5 resolution) were collected on the W01B-MX2 beamline at the Laborat\u00f3rio Nacional de Luz S\u00edncrotron (LNLS, Brazilian Synchrotron Light Laboratory\/MCT). Both crystals belonged to the hexagonal space group<jats:italic>P<\/jats:italic>6<jats:sub>1<\/jats:sub>22, with similar unit-cell parameters<jats:italic>a<\/jats:italic>=<jats:italic>b<\/jats:italic>= 108.5,<jats:italic>c<\/jats:italic>= 55.8\u2005\u00c5. SIRAS-derived phases were used to generate the first electron-density map, from which a partial three-dimensional model of Sm21.7 (from Gln89 to Asn184) was automatically constructed. Anaysis of dissolved crystals by SDS\u2013PAGE confirmed that the protein was cleaved in the crystallization drop and only the Sm21.7 C-terminal domain was crystallized. The structure of the Sm21.7 C-terminal domain will help in the localization of the epitopes responsible for its protective immune responses, constituting important progress in the development of an anti-schistosomiasis vaccine.<\/jats:p>","DOI":"10.1107\/s2053230x14009273","type":"journal-article","created":{"date-parts":[[2014,5,23]],"date-time":"2014-05-23T17:11:45Z","timestamp":1400865105000},"page":"803-807","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":7,"title":["Preliminary crystallographic studies of a<i>Schistosoma mansoni<\/i>antigen (Sm21.7) dynein light-chain (DLC) domain"],"prefix":"10.1107","volume":"70","author":[{"given":"M. A. F.","family":"Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. T. G.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. C. 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