{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:11:45Z","timestamp":1758399105776,"version":"3.33.0"},"reference-count":16,"publisher":"International Union of Crystallography (IUCr)","issue":"10","license":[{"start":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T00:00:00Z","timestamp":1411603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T00:00:00Z","timestamp":1411603200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,10,1]]},"abstract":"<jats:p>Interleukin-18 (IL-18), a pro-inflammatory cytokine belonging to the interleukin-1 (IL-1) family, is involved in the pathogenesis of autoimmune\/autoinflammatory and allergic diseases such as juvenile idiopathic arthritis and bronchial asthma. IL-18 forms a signalling complex with the IL-18 receptor \u03b1 (IL-18R\u03b1) and \u03b2 (IL-18R\u03b2) chains; however, the detailed activation mechanism remains unclear. Here, the IL-18\u2013IL-18R\u03b1 binary and IL-18\u2013IL-18R\u03b1\u2013IL-18R\u03b2 ternary complexes were purified and crystallized as well as IL-18 alone. An X-ray diffraction data set for IL-18 was collected to 2.33\u2005\u00c5 resolution from a crystal belonging to space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 68.15,<jats:italic>b<\/jats:italic>= 79.51,<jats:italic>c<\/jats:italic>= 73.46\u2005\u00c5, \u03b2 = 100.97\u00b0. Crystals of both the IL-18 binary and ternary complexes belonging to the orthorhombic space groups<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2 and<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>, respectively, diffracted to 3.10\u2005\u00c5 resolution. Unit-cell parameters were determined as<jats:italic>a<\/jats:italic>= 135.49,<jats:italic>b<\/jats:italic>= 174.81,<jats:italic>c<\/jats:italic>= 183.40\u2005\u00c5 for the binary complex and<jats:italic>a<\/jats:italic>= 72.56,<jats:italic>b<\/jats:italic>= 111.56,<jats:italic>c<\/jats:italic>= 134.57\u2005\u00c5 for the ternary complex.<\/jats:p>","DOI":"10.1107\/s2053230x14016926","type":"journal-article","created":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T15:45:59Z","timestamp":1411573559000},"page":"1351-1356","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":9,"title":["Purification, crystallization and preliminary X-ray crystallographic analysis of human IL-18 and its extracellular complexes"],"prefix":"10.1107","volume":"70","author":[{"given":"Takeshi","family":"Kimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naotaka","family":"Tsutsumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyohei","family":"Arita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariko","family":"Ariyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Ohnishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Shirakawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zenichiro","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehito","family":"Tochio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,9,25]]},"reference":[{"key":"rl5077_bb1","first-page":"289","volume":"4","author":"Dinarello","year":"2013","journal-title":"Frontiers Immunol."},{"key":"rl5077_bb2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1107\/S0907444905036693","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Cryst. 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Immunol."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/10\/00\/rl5077\/rl5077.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/10\/00\/rl5077\/rl5077.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T09:55:19Z","timestamp":1736762119000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X14016926"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,25]]},"references-count":16,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2014,10]]}},"alternative-id":["S2053230X14016926"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x14016926","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2014,9,25]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","name":"journal","label":"Journal","explanation":{"URL":"http:\/\/journals.iucr.org\/f"}},{"value":"crystallization communications","name":"content_type","label":"Content type","explanation":{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2011\/01\/00\/me0435\/index.html"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"10 June 2014","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"22 July 2014","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"25 September 2014","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2014 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}