{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:15:42Z","timestamp":1790061342914,"version":"4.0.1"},"reference-count":21,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T00:00:00Z","timestamp":1409270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T00:00:00Z","timestamp":1409270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>The<jats:italic>Rv2416c<\/jats:italic>gene of<jats:italic>Mycobacterium tuberculosis<\/jats:italic>(<jats:italic>Mtb<\/jats:italic>) encodes the enhanced intracellular survival (Eis) protein that enhances intracellular survival of the pathogen in host macrophages during infection. The<jats:italic>Mtb<\/jats:italic>Eis protein is released into the cytoplasm of the phagocyte during intracellular infection and modulates the host immune response. It also contributes to drug resistance by acetylating multiple amine groups of aminoglycosides. Interestingly, the nonpathogenic<jats:italic>M. smegmatis<\/jats:italic>(<jats:italic>Msm<\/jats:italic>) contains a homologous<jats:italic>eis<\/jats:italic>gene (MSMEG_3513). The overall structures of<jats:italic>Mtb<\/jats:italic>Eis and<jats:italic>Msm<\/jats:italic>Eis are highly similar to each other, reflecting the high level (58%) of amino-acid sequence identity between them. Both<jats:italic>Mtb<\/jats:italic>Eis and<jats:italic>Msm<\/jats:italic>Eis are active as aminoglycoside acetyltransferases, while only<jats:italic>Mtb<\/jats:italic>Eis functions as an<jats:italic>N<\/jats:italic><jats:sup>\u220a<\/jats:sup>-acetyltransferase to acetylate Lys55 of dual-specificity protein phosphatase 16 (DUSP16)\/mitogen-activated protein kinase phosphatase 7 (MKP-7), leading to the suppression of host immune responses. Here, the crystal structure of<jats:italic>Msm<\/jats:italic>Eis in the paromomycin-bound form is reported, revealing detailed interactions between an aminoglycoside antibiotic and<jats:italic>Msm<\/jats:italic>Eis. The crystal structure of<jats:italic>Msm<\/jats:italic>Eis in the paromomycin-bound form has been determined at 3.3\u2005\u00c5 resolution. This work provides potentially useful information for structure-guided discovery of Eis inhibitors as a novel antituberculosis drug against drug-resistant<jats:italic>Mtb<\/jats:italic>.<\/jats:p>","DOI":"10.1107\/s2053230x14017385","type":"journal-article","created":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T13:09:32Z","timestamp":1409317772000},"page":"1173-1179","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":10,"title":["Structure of<i>Mycobacterium smegmatis<\/i>Eis in complex with paromomycin"],"prefix":"10.1107","volume":"70","author":[{"given":"Kyoung Hoon","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doo Ri","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Jin","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Kuk","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Won","family":"Suh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2014,8,29]]},"reference":[{"key":"tt5057_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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