{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T02:24:59Z","timestamp":1772763899883,"version":"3.50.1"},"reference-count":18,"publisher":"International Union of Crystallography (IUCr)","issue":"10","license":[{"start":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T00:00:00Z","timestamp":1411603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T00:00:00Z","timestamp":1411603200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,10,1]]},"abstract":"<jats:p>Brown spider envenomation results in dermonecrosis, intravascular coagulation, haemolysis and renal failure, mainly owing to the action of sphingomyelinases D (SMases D), which catalyze the hydrolysis of sphingomyelin to produce ceramide 1-phosphate and choline or the hydrolysis of lysophosphatidylcholine to produce lysophosphatidic acid. Here, the heterologous expression, purification, crystallization and preliminary X-ray diffraction analysis of LgRec1, a novel SMase D from<jats:italic>Loxosceles gaucho<\/jats:italic>venom, are reported. The crystals belonged to space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2, with unit-cell parameters<jats:italic>a<\/jats:italic>= 52.98,<jats:italic>b<\/jats:italic>= 62.27,<jats:italic>c<\/jats:italic>= 84.84\u2005\u00c5 and diffracted to a maximum resolution of 2.6\u2005\u00c5.<\/jats:p>","DOI":"10.1107\/s2053230x14019207","type":"journal-article","created":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T15:45:59Z","timestamp":1411573559000},"page":"1418-1420","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":11,"title":["Crystallization and preliminary X-ray diffraction analysis of a novel sphingomyelinase D from<i>Loxosceles gaucho<\/i>venom"],"prefix":"10.1107","volume":"70","author":[{"given":"Anwar","family":"Ullah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geraldo Santana","family":"Magalh\u00e3es","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rehana","family":"Masood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Barros","family":"Mariutti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika Aparecida","family":"Coronado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rio Tyago","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia Cristina","family":"Barbaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raghuvir Krishnaswamy","family":"Arni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,9,25]]},"reference":[{"key":"ub5063_bb1","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1126\/science.3983643","volume":"228","author":"Bernheimer","year":"1985","journal-title":"Science"},{"key":"ub5063_bb2","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.toxicon.2004.11.011","volume":"45","author":"Binford","year":"2005","journal-title":"Toxicon"},{"key":"ub5063_bb3","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/j.bbrc.2011.05.053","volume":"409","author":"Giuseppe","year":"2011","journal-title":"Biochem. 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