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Suppressor\/enhancer of lin-12-like (SEL1L), a component of the dislocation machinery containing the E3 ubiquitin ligase Hrd1, plays an important role in selecting and transporting ERAD substrates for degradation in the endoplasmic reticulum. In this study, the purification, crystallization and preliminary X-ray diffraction analysis of recombinant mouse SEL1L (residues 348\u2013533) are reported. The crystals were obtained by the hanging-drop vapour-diffusion method at pH 8.5 and 277\u2005K using 30% 2-propanol as a precipitant. Optimized crystals diffracted to 3.3\u2005\u00c5 resolution at a synchrotron-radiation source. Preliminary X-ray diffraction analysis revealed that the crystals belonged to space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>and contained four molecules per asymmetric unit, with a solvent content of 44%.<\/jats:p>","DOI":"10.1107\/s2053230x14023115","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T16:19:03Z","timestamp":1415895543000},"page":"1624-1627","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystallization and preliminary X-ray diffraction analysis of the Sel1-like repeats of SEL1L"],"prefix":"10.1107","volume":"70","author":[{"given":"Hanbin","family":"Jeong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hakbong","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changwook","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,11,14]]},"reference":[{"key":"ft5052_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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