{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T08:05:28Z","timestamp":1761984328103,"version":"build-2065373602"},"reference-count":17,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2014,11,14]],"date-time":"2014-11-14T00:00:00Z","timestamp":1415923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,11,14]],"date-time":"2014-11-14T00:00:00Z","timestamp":1415923200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p><jats:italic>Helicobacter pylori<\/jats:italic>is a human pathogen that colonizes about 50% of the world's population, causing chronic gastritis, duodenal ulcers and even gastric cancer. A steady emergence of multiple antibiotic resistant strains poses an important public health threat and there is an urgent requirement for alternative therapeutics. The blood group antigen-binding adhesin BabA mediates the intimate attachment to the host mucosa and forms a major candidate for novel vaccine and drug development. Here, the recombinant expression and crystallization of a soluble BabA truncation (BabA<jats:sup>25\u2013460<\/jats:sup>) corresponding to the predicted extracellular adhesin domain of the protein are reported. X-ray diffraction data for nanobody-stabilized BabA<jats:sup>25\u2013460<\/jats:sup>were collected to 2.25\u2005\u00c5 resolution from a crystal that belonged to space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 50.96,<jats:italic>b<\/jats:italic>\u00a0= 131.41,<jats:italic>c<\/jats:italic>= 123.40\u2005\u00c5, \u03b1 = 90.0, \u03b2 = 94.8, \u03b3 = 90.0\u00b0, and which was predicted to contain two BabA<jats:sup>25\u2013460<\/jats:sup>\u2013nanobody complexes per asymmetric unit.<\/jats:p>","DOI":"10.1107\/s2053230x14023188","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T16:19:03Z","timestamp":1415895543000},"page":"1631-1635","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":10,"title":["Expression, purification and X-ray crystallographic analysis of the<i>Helicobacter pylori<\/i>blood group antigen-binding adhesin BabA"],"prefix":"10.1107","volume":"70","author":[{"given":"Suresh","family":"Subedi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristof","family":"Moonens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ema","family":"Rom\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvin","family":"Lo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Vandenbussche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanna","family":"Bugaytsova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serge","family":"Muyldermans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Bor\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Remaut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,11,14]]},"reference":[{"key":"us5068_bb1","doi-asserted-by":"crossref","first-page":"4155","DOI":"10.1128\/IAI.68.7.4155-4168.2000","volume":"68","author":"Alm","year":"2000","journal-title":"Infect. 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