{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T08:48:29Z","timestamp":1736844509174,"version":"3.33.0"},"reference-count":24,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T00:00:00Z","timestamp":1417132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T00:00:00Z","timestamp":1417132800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>Melioidosis is an infectious disease caused by the pathogenic bacterium<jats:italic>Burkholderia pseudomallei<\/jats:italic>. Whole-genome sequencing revealed that the<jats:italic>B. pseudomallei<\/jats:italic>genome includes 5855 coding DNA sequences (CDSs), of which \u223c25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in<jats:italic>Escherichia coli<\/jats:italic>, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55\u2005\u00c5 resolution. It belonged to space group<jats:italic>C<\/jats:italic>222<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 85.36,<jats:italic>b<\/jats:italic>= 115.63,<jats:italic>c<\/jats:italic>= 46.73\u2005\u00c5. The calculated Matthews coefficient (<jats:italic>V<\/jats:italic><jats:sub>M<\/jats:sub>) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%.<\/jats:p>","DOI":"10.1107\/s2053230x14025278","type":"journal-article","created":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T19:18:01Z","timestamp":1417202281000},"page":"1697-1700","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from<i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1107","volume":"70","author":[{"given":"Sofiyah","family":"Shaibullah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurhikmah","family":"Mohd-Sharif","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kok Lian","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd","family":"Firdaus-Raih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila","family":"Nathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahmah","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chyan Leong","family":"Ng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2014,11,28]]},"reference":[{"key":"us5069_bb1","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"us5069_bb2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1107\/S0907444910048675","volume":"67","author":"Battye","year":"2011","journal-title":"Acta Cryst. D"},{"key":"us5069_bb3","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1128\/IAI.01218-10","volume":"79","author":"Burtnick","year":"2011","journal-title":"Infect. Immun."},{"key":"us5069_bb4","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","volume":"18","author":"Cheng","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"us5069_bb5","unstructured":"Crowther, R. A. (1972). The Molecular Replacement Method, edited by M. G. Rossmann, pp. 173-178. New York: Gordon & Breach."},{"key":"us5069_bb6","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1126\/science.1211915","volume":"334","author":"Cruz-Migoni","year":"2011","journal-title":"Science"},{"key":"us5069_bb8","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.cell.2014.02.017","volume":"156","author":"Daumke","year":"2014","journal-title":"Cell"},{"key":"us5069_bb9","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1107\/S0907444905036693","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Cryst. D"},{"key":"us5069_bb10","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1107\/S0907444913000061","volume":"69","author":"Evans","year":"2013","journal-title":"Acta Cryst. D"},{"key":"us5069_bb11","doi-asserted-by":"crossref","first-page":"6604","DOI":"10.1128\/JB.01577-12","volume":"194","author":"Fang","year":"2012","journal-title":"J. Bacteriol."},{"key":"us5069_bb13","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1099\/mic.0.046540-0","volume":"157","author":"Gamage","year":"2011","journal-title":"Microbiology"},{"key":"us5069_bb14","unstructured":"Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M. R., Appel, R. D. & Bairoch, A. (2005). The Proteomics Protocols Handbook, edited by J. M. Walker, pp. 571-607. Totawa: Humana Press."},{"key":"us5069_bb15","doi-asserted-by":"crossref","first-page":"14240","DOI":"10.1073\/pnas.0403302101","volume":"101","author":"Holden","year":"2004","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"us5069_bb16","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1128\/CVI.00021-13","volume":"20","author":"James","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"us5069_bb17","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/nprot.2009.2","volume":"4","author":"Kelley","year":"2009","journal-title":"Nature Protoc."},{"key":"us5069_bb18","doi-asserted-by":"crossref","first-page":"e94981","DOI":"10.1371\/journal.pone.0094981","volume":"11","author":"Lassaux","year":"2014","journal-title":"PLoS One"},{"key":"us5069_bb19","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1093\/nar\/gks1243","volume":"41","author":"Marchler-Bauer","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"us5069_bb20","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3201\/eid0802.010164","volume":"8","author":"Rotz","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"us5069_bb21","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.2217\/fmb.12.116","volume":"7","author":"Schweizer","year":"2012","journal-title":"Future Microbiol."},{"key":"us5069_bb22","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1046\/j.1365-2958.2002.03190.x","volume":"46","author":"Stevens","year":"2002","journal-title":"Mol. Microbiol."},{"key":"us5069_bb23","doi-asserted-by":"crossref","first-page":"e00656","DOI":"10.1128\/genomeA.00656-13","volume":"1","author":"Stone","year":"2013","journal-title":"Genome Announc."},{"key":"us5069_bb24","doi-asserted-by":"crossref","first-page":"4693","DOI":"10.1038\/srep04693","volume":"4","author":"Suarez","year":"2014","journal-title":"Sci. Rep."},{"key":"us5069_bb25","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1107\/S0907444909042589","volume":"66","author":"Vagin","year":"2010","journal-title":"Acta Cryst. D"},{"key":"us5069_bb26","first-page":"803","volume":"22","author":"Winsor","year":"2008","journal-title":"Bioinformatics"}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/12\/00\/us5069\/us5069.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2014\/12\/00\/us5069\/us5069.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T10:03:51Z","timestamp":1736762631000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X14025278"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,28]]},"references-count":24,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2014,12]]}},"alternative-id":["S2053230X14025278"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x14025278","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2014,11,28]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","name":"journal","label":"Journal","explanation":{"URL":"http:\/\/journals.iucr.org\/f"}},{"value":"crystallization communications","name":"content_type","label":"Content type","explanation":{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2011\/01\/00\/me0435\/index.html"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"22 September 2014","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"18 November 2014","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"28 November 2014","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"http:\/\/scripts.iucr.org\/cgi-bin\/sendsup?us5069"}},{"value":"\u00a9 2014 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}