{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T08:15:59Z","timestamp":1761984959420,"version":"build-2065373602"},"reference-count":11,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T00:00:00Z","timestamp":1422403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T00:00:00Z","timestamp":1422403200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"<jats:p><jats:italic>Vibrio cholerae<\/jats:italic>, the causative agent of cholera, has developed a variety of mechanisms to obtain the limited-availability iron from human hosts. One important method for iron acquisition is through haem-uptake systems. Although the transport of haem has been widely studied, the fate of haem once it enters the cytoplasm remains an open question. Here, preliminary X-ray crystallographic analysis was performed on HutX, a member of the conserved haem-utilization operon from<jats:italic>V. cholerae<\/jats:italic>strain N16961. The crystals of HutX were found to belong to the orthorhombic space group<jats:italic>C<\/jats:italic>222<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 50.1,<jats:italic>b<\/jats:italic>= 169.0,<jats:italic>c<\/jats:italic>= 81.8\u2005\u00c5. There are two protein molecules in the asymmetric unit, with a corresponding Matthews coefficient<jats:italic>V<\/jats:italic><jats:sub>M<\/jats:sub>of 2.06\u2005\u00c5<jats:sup>3<\/jats:sup>\u2005Da<jats:sup>\u22121<\/jats:sup>and a solvent content of 40.28%.<\/jats:p>","DOI":"10.1107\/s2053230x14027666","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T16:32:23Z","timestamp":1422376343000},"page":"141-144","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Expression, purification and preliminary crystallographic analysis of a haem-utilizing protein, HutX, from<i>Vibrio cholerae<\/i>"],"prefix":"10.1107","volume":"71","author":[{"given":"Tiantian","family":"Su","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaikai","family":"Chi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liming","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2015,1,28]]},"reference":[{"key":"rl5084_bb1","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/j.1365-2958.1993.tb01137.x","volume":"7","author":"Henderson","year":"1993","journal-title":"Mol. 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Bacteriol."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2015\/02\/00\/rl5084\/rl5084.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2015\/02\/00\/rl5084\/rl5084.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T10:10:46Z","timestamp":1736763046000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X14027666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,28]]},"references-count":11,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,2]]}},"alternative-id":["S2053230X14027666"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x14027666","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2015,1,28]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","name":"journal","label":"Journal","explanation":{"URL":"http:\/\/journals.iucr.org\/f"}},{"value":"research communications","name":"content_type","label":"Content type","explanation":{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2011\/01\/00\/me0435\/index.html"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"8 September 2014","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"18 December 2014","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"28 January 2015","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2015 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}