{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T12:50:22Z","timestamp":1775393422167,"version":"3.50.1"},"reference-count":18,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T00:00:00Z","timestamp":1440460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T00:00:00Z","timestamp":1440460800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2015,9,1]]},"abstract":"<jats:p>Nonstructural protein 15 (nsp15), also called endoribonuclease, is a gene product of open reading frame 1b (ORF 1b) in coronaviruses. It is an important enzyme in the transcription\/replication process involved in discontinuous negative-strand RNA synthesis. In this work, mutants of nsp15 from<jats:italic>Human coronavirus 229E<\/jats:italic>(HCoV-229E) were made based on structural analysis of the homologous nsp15s in<jats:italic>Severe acute respiratory syndrome coronavirus<\/jats:italic>(SARS-CoV) and<jats:italic>Mouse hepatitis virus<\/jats:italic>(MHV). The I26A\/N52A mutant of nsp15 was overexpressed, purified and crystallized, and this mutant led to a trimeric form rather than hexamers or monomers. Crystals of trimeric nsp15 were obtained by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant and diffracted to 2.5\u2005\u00c5 resolution. The crystals belonged to space group<jats:italic>C<\/jats:italic>222<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 85.9,<jats:italic>b<\/jats:italic>= 137.5,<jats:italic>c<\/jats:italic>= 423.1\u2005\u00c5, \u03b1 = \u03b2 = \u03b3 = 90\u00b0.<\/jats:p>","DOI":"10.1107\/s2053230x15007359","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T15:31:25Z","timestamp":1440516685000},"page":"1156-1160","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":6,"title":["Crystallization and preliminary X-ray crystallographic analysis of a nonstructural protein 15 mutant from<i>Human coronavirus 229E<\/i>"],"prefix":"10.1107","volume":"71","author":[{"given":"Tong","family":"Huo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2015,8,25]]},"reference":[{"key":"hc5195_bb1","doi-asserted-by":"crossref","first-page":"12218","DOI":"10.1128\/JVI.78.22.12218-12224.2004","volume":"78","author":"Bhardwaj","year":"2004","journal-title":"J. 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