{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T07:53:20Z","timestamp":1779350000504,"version":"3.51.4"},"reference-count":35,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T00:00:00Z","timestamp":1440460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T00:00:00Z","timestamp":1440460800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2015,9,1]]},"abstract":"<jats:p>Co-crystals of the bovine Arp2\/3 complex with the CA motif from N-WASP in two new space groups were analyzed by X-ray diffraction. The crystals in the orthorhombic space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>contained one complex per asymmetric unit, with unit-cell parameters<jats:italic>a<\/jats:italic>= 105.48,<jats:italic>b<\/jats:italic>= 156.71,<jats:italic>c<\/jats:italic>= 177.84\u2005\u00c5, and diffracted to 3.9\u2005\u00c5 resolution. The crystals in the tetragonal space group<jats:italic>P<\/jats:italic>4<jats:sub>1<\/jats:sub>contained two complexes per asymmetric unit, with unit-cell parameters<jats:italic>a<\/jats:italic>=<jats:italic>b<\/jats:italic>= 149.93,<jats:italic>c<\/jats:italic>\u00a0=\u00a0265.91\u2005\u00c5, and diffracted to 5.0\u2005\u00c5 resolution. The electron-density maps of both new crystal forms had densities for small segments of subdomains 1 and 2 of Arp2. Both maps had density at the binding site on Arp3 for the C-terminal EWE tripeptide from N-WASP and a binding site proposed for the C motif of N-WASP in the barbed-end groove of Arp2. The map from the tetragonal crystal form had density near the barbed end of Arp3 that may correspond to the C helix of N-WASP. The noise levels and the low resolution of the maps made the assignment of specific molecular structures for any of these CA peptides impossible.<\/jats:p>","DOI":"10.1107\/s2053230x15013515","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T15:31:25Z","timestamp":1440516685000},"page":"1161-1168","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":10,"title":["Crystals of the Arp2\/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites"],"prefix":"10.1107","volume":"71","author":[{"given":"Christopher T.","family":"Jurgenson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas D.","family":"Pollard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2015,8,25]]},"reference":[{"key":"hv5302_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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