{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T10:30:55Z","timestamp":1769077855227,"version":"3.49.0"},"reference-count":18,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T00:00:00Z","timestamp":1447804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T00:00:00Z","timestamp":1447804800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2015,12,1]]},"abstract":"<jats:p>Plant polyketides are a structurally diverse family of natural products. In the biosynthesis of plant polyketides, the construction of the carbocyclic scaffold is a key step in diversifying the polyketide structure. Olivetolic acid cyclase (OAC) from<jats:italic>Cannabis sativa<\/jats:italic>L. is the only known plant polyketide cyclase that catalyzes the C2\u2013C7 intramolecular aldol cyclization of linear pentyl tetra-\u03b2-ketide-CoA to generate olivetolic acid in the biosynthesis of cannabinoids. The enzyme is also thought to belong to the dimeric \u03b1+\u03b2 barrel (DABB) protein family. However, because of a lack of functional analysis of other plant DABB proteins and low sequence identity with the functionally distinct bacterial DABB proteins, the catalytic mechanism of OAC has remained unclear. To clarify the intimate catalytic mechanism of OAC, the enzyme was overexpressed in<jats:italic>Escherichia coli<\/jats:italic>and crystallized using the vapour-diffusion method. The crystals diffracted X-rays to 1.40\u2005\u00c5 resolution and belonged to space group<jats:italic>P<\/jats:italic>3<jats:sub>1<\/jats:sub>21 or<jats:italic>P<\/jats:italic>3<jats:sub>2<\/jats:sub>21, with unit-cell parameters<jats:italic>a<\/jats:italic>=<jats:italic>b<\/jats:italic>= 47.3,<jats:italic>c<\/jats:italic>= 176.0\u2005\u00c5. Further crystallographic analysis will provide valuable insights into the structure\u2013function relationship and catalytic mechanism of OAC.<\/jats:p>","DOI":"10.1107\/s2053230x15020385","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T16:21:08Z","timestamp":1447777268000},"page":"1470-1474","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":7,"title":["Expression, purification and crystallization of a plant polyketide cyclase from<i>Cannabis sativa<\/i>"],"prefix":"10.1107","volume":"71","author":[{"given":"Xinmei","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Matsui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Futoshi","family":"Taura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Noguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuro","family":"Abe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Morita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2015,11,18]]},"reference":[{"key":"tb5091_bb1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.phytochem.2013.01.001","volume":"88","author":"Abdel-Rahman","year":"2013","journal-title":"Phytochemistry"},{"key":"tb5091_bb2","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1039\/b909988n","volume":"27","author":"Abe","year":"2010","journal-title":"Nat. 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