{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T12:36:30Z","timestamp":1753274190889,"version":"3.33.0"},"reference-count":16,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T00:00:00Z","timestamp":1447804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T00:00:00Z","timestamp":1447804800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2015,12,1]]},"abstract":"<jats:p>The prokaryotic obligate intracellular pathogen<jats:italic>Chlamydia trachomatis<\/jats:italic>is the most prevalent cause of preventable blindness, affecting approximately six million people worldwide. In addition,<jats:italic>C. trachomatis<\/jats:italic>is the most commonly reported sexually transmitted pathogen in Europe and the US, causing pelvic inflammation, ectopic pregnancy and infertility. As in other intracellular pathogens, proteases play crucial roles during most stages of the complex life cycle of<jats:italic>Chlamydia<\/jats:italic>. CT441 is a chlamydial protease that has been reported to interfere with oestrogen signalling of the host cell. Here, the recombinant production, purification and crystallization of an inactive variant of CT441, designated CT441\u00b0 (active-site Ser455 replaced by Ala), are described. CT441\u00b0 was crystallized in space group<jats:italic>P<\/jats:italic>22<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 86.7,<jats:italic>b<\/jats:italic>= 184.0,<jats:italic>c<\/jats:italic>= 209.6\u2005\u00c5. A complete diffraction data set was collected to a resolution of 2.95\u2005\u00c5.<\/jats:p>","DOI":"10.1107\/s2053230x15020518","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T16:21:08Z","timestamp":1447777268000},"page":"1454-1458","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Production, crystallization and X-ray diffraction analysis of the protease CT441 from<i>Chlamydia trachomatis<\/i>"],"prefix":"10.1107","volume":"71","author":[{"given":"Friedrich","family":"Kohlmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kensuke","family":"Shima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Rupp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Solbach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"Hilgenfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2015,11,18]]},"reference":[{"key":"no5097_bb1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jsbmb.2010.01.004","volume":"119","author":"Borth","year":"2010","journal-title":"J. Steroid Biochem. Mol. Biol."},{"unstructured":"","key":"no5097_bb2"},{"key":"no5097_bb3","doi-asserted-by":"crossref","first-page":"e1002842","DOI":"10.1371\/journal.ppat.1002842","volume":"8","author":"Chen","year":"2012","journal-title":"PLoS Pathog."},{"key":"no5097_bb4","doi-asserted-by":"crossref","first-page":"41320","DOI":"10.1074\/jbc.M110.152280","volume":"285","author":"Christian","year":"2010","journal-title":"J. Biol. Chem."},{"unstructured":"","key":"no5097_bb5"},{"key":"no5097_bb6","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1107\/S0907444905036693","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Cryst. D"},{"key":"no5097_bb8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"no5097_bb9","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1128\/JB.02140-14","volume":"197","author":"Kohlmann","year":"2015","journal-title":"J. Bacteriol."},{"key":"no5097_bb10","doi-asserted-by":"crossref","first-page":"2933","DOI":"10.1073\/pnas.0608393104","volume":"104","author":"Lad","year":"2007","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"no5097_bb11","doi-asserted-by":"crossref","first-page":"6619","DOI":"10.1128\/JB.00429-07","volume":"189","author":"Lad","year":"2007","journal-title":"J. Bacteriol."},{"key":"no5097_bb12","doi-asserted-by":"crossref","first-page":"D225","DOI":"10.1093\/nar\/gkq1189","volume":"39","author":"Marchler-Bauer","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"no5097_bb13","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1136\/bjo.2008.148494","volume":"93","author":"Mariotti","year":"2009","journal-title":"Br. J. Ophthalmol."},{"key":"no5097_bb14","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0022-2836(68)90205-2","volume":"33","author":"Matthews","year":"1968","journal-title":"J. Mol. Biol."},{"key":"no5097_bb15","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1107\/S0909049512006395","volume":"19","author":"Mueller","year":"2012","journal-title":"J. Synchrotron Rad."},{"key":"no5097_bb16","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1056\/NEJMcp030542","volume":"349","author":"Peipert","year":"2003","journal-title":"N. Engl. J. Med."},{"key":"no5097_bb17","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1107\/S0021889897003907","volume":"30","author":"Weiss","year":"1997","journal-title":"J. Appl. Cryst."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2015\/12\/00\/no5097\/no5097.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2015\/12\/00\/no5097\/no5097.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T10:17:33Z","timestamp":1736763453000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X15020518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,18]]},"references-count":16,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2015,12]]}},"alternative-id":["S2053230X15020518"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x15020518","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2015,11,18]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/f"}},{"value":"research communications","name":"content_type","label":"Content type","explanation":{"URL":"http:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"29 September 2015","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"29 October 2015","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"18 November 2015","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2015 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}