{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T17:42:06Z","timestamp":1762623726817,"version":"3.33.0"},"reference-count":23,"publisher":"International Union of Crystallography (IUCr)","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:p>Nepenthesins are aspartic proteases secreted by carnivorous pitcher plants of the genus<jats:italic>Nepenthes<\/jats:italic>. They significantly differ in sequence from other plant aspartic proteases. This difference, which provides more cysteine residues in the structure of nepenthesins, may contribute to their unique stability profile. Recombinantly produced nepenthesin 1 (rNep1) from<jats:italic>N. gracilis<\/jats:italic>in complex with pepstatin A was crystallized under two different crystallization conditions using a newly formulated low-pH crystallization screen. The diffraction data were processed to 2.9 and 2.8\u2005\u00c5 resolution, respectively. The crystals belonged to space group<jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>2<jats:sub>1<\/jats:sub>, with unit-cell parameters<jats:italic>a<\/jats:italic>= 86.63,<jats:italic>b<\/jats:italic>= 95.90,<jats:italic>c<\/jats:italic>= 105.40\u2005\u00c5, \u03b1 = \u03b2 = \u03b3 = 90\u00b0 and<jats:italic>a<\/jats:italic>= 86.28,<jats:italic>b<\/jats:italic>= 97.22,<jats:italic>c<\/jats:italic>= 103.78\u2005\u00c5, \u03b1 = \u03b2 = \u03b3 = 90\u00b0, respectively. Matthews coefficient and solvent-content calculations suggest the presence of two molecules of rNep1 in the asymmetric unit. Here, the details of the crystallization experiment and analysis of the X-ray data are reported.<\/jats:p>","DOI":"10.1107\/s2053230x15022323","type":"journal-article","created":{"date-parts":[[2015,12,13]],"date-time":"2015-12-13T05:41:00Z","timestamp":1449985260000},"page":"24-28","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":5,"title":["Crystallization of nepenthesin I using a low-pH crystallization screen"],"prefix":"10.1107","volume":"72","author":[{"given":"Karla","family":"Fejfarov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"K\u00e1dek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hynek","family":"Mr\u00e1zek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u0159\u00ed","family":"Hausner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vyacheslav","family":"Tretyachenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Koval'","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Man","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jind\u0159ich","family":"Ha\u0161ek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Dohn\u00e1lek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2016,1,1]]},"reference":[{"key":"nj5241_bb1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1042\/BJ20031575","volume":"381","author":"Athauda","year":"2004","journal-title":"Biochem. 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