{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T11:03:41Z","timestamp":1761995021553,"version":"build-2065373602"},"reference-count":20,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T00:00:00Z","timestamp":1484784000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T00:00:00Z","timestamp":1484784000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"published-print":{"date-parts":[[2017,2,1]]},"abstract":"<jats:p>The Gram-negative bacterium<jats:italic>Burkholderia pseudomallei<\/jats:italic>is the causative agent of melioidosis. D-<jats:italic>glycero<\/jats:italic>-\u03b2-D-<jats:italic>manno<\/jats:italic>-Heptose-1-phosphate adenylyltransferase (HldC) is the fourth enzyme of the ADP-L-<jats:italic>glycero<\/jats:italic>-\u03b2-D-<jats:italic>manno<\/jats:italic>-heptose biosynthesis pathway, which produces an essential carbohydrate comprising the inner core of lipopolysaccharide. Therefore, HldC is a potential target of antibiotics against melioidosis. In this study, HldC from<jats:italic>B. pseudomallei<\/jats:italic>has been cloned, expressed, purified and crystallized. Synchrotron X-ray data from a selenomethionine-substituted HldC crystal were also collected to 2.8\u2005\u00c5 resolution. The crystal belonged to the primitive triclinic space group<jats:italic>P<\/jats:italic>1, with unit-cell parameters<jats:italic>a<\/jats:italic>= 74.0,<jats:italic>b<\/jats:italic>= 74.0,<jats:italic>c<\/jats:italic>= 74.9\u2005\u00c5, \u03b1 = 108.4, \u03b2 = 108.4, \u03b3 = 108.0\u00b0. Eight protomers are present in the unit cell and three out of five selenomethionines were found in each protomer using the<jats:italic>PHENIX<\/jats:italic>software suite. A full structural determination is in progress to elucidate the structure\u2013function relationship of the protein.<\/jats:p>","DOI":"10.1107\/s2053230x16020537","type":"journal-article","created":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T16:24:42Z","timestamp":1484843082000},"page":"90-94","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":3,"title":["Expression and crystallographic studies of<scp>D<\/scp>-<i>glycero<\/i>-\u03b2-<scp>D<\/scp>-<i>manno<\/i>-heptose-1-phosphate adenylyltransferase from<i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1107","volume":"73","author":[{"given":"Jimin","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyojin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suwon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daeun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Hae","family":"Shin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2017,1,19]]},"reference":[{"key":"sw5099_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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