{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:42:28Z","timestamp":1788381748568,"version":"build-2803163510"},"reference-count":39,"publisher":"International Union of Crystallography (IUCr)","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1080784"],"award-info":[{"award-number":["GNT1080784"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2018,11,20]]},"published-print":{"date-parts":[[2019,1,1]]},"abstract":"<jats:p>The glutathione reductase (GR) from<jats:italic>Streptococcus pneumoniae<\/jats:italic>is a flavoenzyme that catalyzes the reduction of oxidized glutathione (GSSG) to its reduced form (GSH) in the cytoplasm of this bacterium. The maintenance of an intracellular pool of GSH is critical for the detoxification of reactive oxygen and nitrogen species and for intracellular metal tolerance to ions such as zinc. Here,<jats:italic>S. pneumoniae<\/jats:italic>GR (<jats:italic>Sp<\/jats:italic>GR) was overexpressed and purified and its crystal structure determined at 2.56\u2005\u00c5 resolution.<jats:italic>Sp<\/jats:italic>GR shows overall structural similarity to other characterized GRs, with a dimeric structure that includes an antiparallel \u03b2-sheet at the dimer interface. This observation, in conjunction with comparisons with the interface structures of other GR enzymes, allows the classification of these enzymes into three classes. Analyses of the kinetic properties of<jats:italic>Sp<\/jats:italic>GR revealed a significantly higher value for<jats:italic>K<\/jats:italic><jats:sub>m(GSSG)<\/jats:sub>(231.2 \u00b1 24.7\u2005\u00b5<jats:italic>M<\/jats:italic>) in comparison to other characterized GR enzymes<jats:italic>.<\/jats:italic><\/jats:p>","DOI":"10.1107\/s2053230x18016527","type":"journal-article","created":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T17:05:08Z","timestamp":1545411908000},"page":"54-61","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":14,"title":["The structure and activity of the glutathione reductase from<i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1107","volume":"75","author":[{"given":"Mwilye","family":"Sikanyika","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Arag\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1596-4841","authenticated-orcid":true,"given":"Christopher A.","family":"McDevitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan J.","family":"Maher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"cb5109_bb39","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1107\/S1600577518003120","volume":"25","author":"Arag\u00e3o","year":"2018","journal-title":"J. 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