{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T08:18:45Z","timestamp":1758874725074,"version":"3.37.3"},"reference-count":24,"publisher":"International Union of Crystallography (IUCr)","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31700672"],"award-info":[{"award-number":["31700672"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2017BC009"],"award-info":[{"award-number":["ZR2017BC009"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003091","name":"Shandong Province","doi-asserted-by":"publisher","award":["J17KA127"],"award-info":[{"award-number":["J17KA127"]}],"id":[{"id":"10.13039\/501100003091","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008853","name":"Jining Medical University","doi-asserted-by":"publisher","award":["4"],"award-info":[{"award-number":["4"]}],"id":[{"id":"10.13039\/501100008853","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2018,11,29]]},"published-print":{"date-parts":[[2019,1,1]]},"abstract":"<jats:p>Human xeroderma pigmentosum complementation group A (XPA) is a scaffold protein that plays significant roles in DNA-damage verification and in recruiting downstream endonucleases to facilitate the repair of DNA lesions in nucleotide-excision repair. XPA<jats:sub>98\u2013219<\/jats:sub> (residues 98\u2013219) has been identified as a DNA-binding domain and has been extensively studied in the last two decades. However, the most recent studies have redefined the DNA-binding domain as XPA<jats:sub>98\u2013239<\/jats:sub> (residues 98\u2013239); it exerts a remarkably higher DNA-binding affinity than XPA<jats:sub>98\u2013219<\/jats:sub> and has a binding affinity that is quite similar to that of the full-length protein. Here, the production, crystallization and structure solution of human XPA<jats:sub>98\u2013239<\/jats:sub> are described. Crystals were obtained using a precipitant composed of 1.8\u2005<jats:italic>M<\/jats:italic> ammonium citrate tribasic pH 7.0. Native X-ray diffraction data and zinc single-wavelength anomalous diffraction (SAD) data were collected to 1.93 and 2.06\u2005\u00c5 resolution, respectively. The crystals belonged to space group <jats:italic>P<\/jats:italic>3, with unit-cell parameters <jats:italic>a<\/jats:italic> = 67.1, <jats:italic>b<\/jats:italic> = 67.1, <jats:italic>c<\/jats:italic> = 35.6\u2005\u00c5, \u03b3\u00a0=\u00a0120.0\u00b0. Crystal-content analysis showed the presence of one molecule in the asymmetric unit, corresponding to a Matthews coefficient of 2.65\u2005\u00c5<jats:sup>3<\/jats:sup>\u2005Da<jats:sup>\u22121<\/jats:sup> and a solvent content of 53.6%. The initial phases were solved and the structure model was automatically built by zinc SAD using the <jats:italic>AutoSol<\/jats:italic> program. The initial structure model covered 119 of 142 residues in the asymmetric unit, with an <jats:italic>R<\/jats:italic>\n                  <jats:sub>work<\/jats:sub> of 22.15% and an <jats:italic>R<\/jats:italic>\n                  <jats:sub>free<\/jats:sub> of 25.82%. Compared with a previously obtained truncated solution NMR structure of XPA (residues 98\u2013210), a 19-residue C-terminal extension (residues 211\u2013229, corresponding to 10 of the 20 extra C-terminal residues in the redefined domain for enhanced DNA binding) was contained in this initial model. Refinement of the atomic coordinates of XPA is ongoing.<\/jats:p>","DOI":"10.1107\/s2053230x18016990","type":"journal-article","created":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T17:05:08Z","timestamp":1545411908000},"page":"62-66","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":5,"title":["The redefined DNA-binding domain of human xeroderma pigmentosum complementation group A: production, crystallization and structure solution"],"prefix":"10.1107","volume":"75","author":[{"given":"Fu-Ming","family":"Lian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangwei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7437-9197","authenticated-orcid":true,"given":"Wancai","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Liang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4491-4421","authenticated-orcid":true,"given":"Chengmin","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"ek5011_bb1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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Biol."},{"key":"ek5011_bb7","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1110\/ps.0350503","volume":"12","author":"Kantardjieff","year":"2003","journal-title":"Protein Sci."},{"key":"ek5011_bb8","doi-asserted-by":"crossref","first-page":"8272","DOI":"10.1073\/pnas.1508509112","volume":"112","author":"Koch","year":"2015","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"ek5011_bb9","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/1750-1172-6-70","volume":"6","author":"Lehmann","year":"2011","journal-title":"Orphanet J. Rare Dis."},{"key":"ek5011_bb10","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/nrm3822","volume":"15","author":"Marteijn","year":"2014","journal-title":"Nature Rev. Mol. Cell Biol."},{"key":"ek5011_bb11","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. 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Cell"},{"key":"ek5011_bb22","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Cryst. D"},{"key":"ek5011_bb23","doi-asserted-by":"crossref","first-page":"15921","DOI":"10.1021\/bi061626q","volume":"45","author":"Yang","year":"2006","journal-title":"Biochemistry"},{"key":"ek5011_bb24","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1083\/jcb.201403096","volume":"206","author":"Ziani","year":"2014","journal-title":"J. Cell Biol."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2019\/01\/00\/ek5011\/ek5011.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2019\/01\/00\/ek5011\/ek5011.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T11:12:28Z","timestamp":1736766748000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X18016990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,1]]},"references-count":24,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1]]}},"alternative-id":["S2053230X18016990"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x18016990","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2019,1,1]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"http:\/\/scripts.iucr.org\/cgi-bin\/citedin?ek5011","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"26 October 2018","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"29 November 2018","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"1 January 2019","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2019 International Union of Crystallography","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}}]}}