{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T13:47:59Z","timestamp":1765979279327,"version":"3.48.0"},"reference-count":37,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022762","name":"Hans-Fischer-Gesellschaft","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022762","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2019,8,4]]},"published-print":{"date-parts":[[2019,9,1]]},"abstract":"<jats:p>\n                    A putative open reading frame encoding GTP cyclohydrolase I from\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    was expressed in a recombinant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    strain. The recombinant protein was purified and was confirmed to convert GTP to dihydroneopterin triphosphate (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    = 53\u2005\u00b5\n                    <jats:italic>M<\/jats:italic>\n                    ;\n                    <jats:italic>v<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    = 180\u2005nmol\u2005mg\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2005min\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). The\u00a0protein was crystallized from 1.3\u2005\n                    <jats:italic>M<\/jats:italic>\n                    sodium citrate pH 7.3 and the crystal structure was solved at a resolution of 2.4\u2005\u00c5 (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>free<\/jats:sub>\n                    = 0.226) by molecular replacement using human GTP cyclohydrolase I as a template. The protein is a\n                    <jats:italic>D<\/jats:italic>\n                    <jats:sub>5<\/jats:sub>\n                    -symmetric decamer with ten topologically equivalent active sites. Screening a small library of about 9000 compounds afforded several inhibitors with IC\n                    <jats:sub>50<\/jats:sub>\n                    values in the low-micromolar range. Several inhibitors had significant selectivity with regard to human GTP cyclohydrolase I. Hence, GTP cyclohydrolase I may be a potential target for novel drugs directed at microbial infections, including listeriosis, a rare disease with high mortality.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x19010902","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T08:28:49Z","timestamp":1567153729000},"page":"586-592","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":5,"title":["Structure of GTP cyclohydrolase I from\n                    <i>Listeria monocytogenes<\/i>\n                    , a potential anti-infective drug target"],"prefix":"10.1107","volume":"75","author":[{"given":"Sonja","family":"Sch\u00fcssler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilka","family":"Haase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Perbandt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Illarionov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Siemens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Richter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adelbert","family":"Bacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Gr\u00e4wert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"no5161_bb1","doi-asserted-by":"crossref","first-page":"13567","DOI":"10.1073\/pnas.240463497","volume":"97","author":"Auerbach","year":"2000","journal-title":"Proc. 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