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The \u03b2-barrel assembly machinery (BAM) complex is one of the OMPs of <jats:italic>Borrelia burgdorferi<\/jats:italic>, the pathogenic spirochete that causes Lyme disease, and its BamA component (<jats:italic>Bb<\/jats:italic>BamA) includes a C-terminal \u03b2-barrel domain and five N-terminal periplasmic polypeptide-transport-associated (POTRA) domains, which together perform a central transport function. In the current work, the production, crystallization and X-ray analysis of the three N-terminal POTRA domains of <jats:italic>Bb<\/jats:italic>BamA (<jats:italic>Bb<\/jats:italic>BamA-POTRA P1\u2013P3; residues 30\u2013273) were carried out. The crystals of <jats:italic>Bb<\/jats:italic>BamA-POTRA P1\u2013P3 belonged to space group <jats:italic>P<\/jats:italic>2<jats:sub>1<\/jats:sub>, with unit-cell parameters <jats:italic>a<\/jats:italic> = 45.353, <jats:italic>b<\/jats:italic> = 111.538, <jats:italic>c<\/jats:italic> = 64.376\u2005\u00c5, \u03b2 = 99.913\u00b0. The Matthews coefficient was calculated to be 2.92\u2005\u00c5<jats:sup>3<\/jats:sup>\u2005Da<jats:sup>\u22121<\/jats:sup>, assuming the presence of two molecules per asymmetric unit, and the corresponding solvent content was 57.9%. Owing to the absence of an ideal homology model, numerous attempts to solve the <jats:italic>Bb<\/jats:italic>BamA-POTRA P1\u2013P3 structure using molecular replacement (MR) failed. In order to solve the structure, further trials using selenomethionine derivatization are currently being carried out.<\/jats:p>","DOI":"10.1107\/s2053230x20006196","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T15:23:33Z","timestamp":1590765813000},"page":"235-240","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Crystallization and X-ray analysis of <i>Borrelia burgdorferi<\/i> \u03b2-barrel assembly machinery A"],"prefix":"10.1107","volume":"76","author":[{"given":"Shishang","family":"Dong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongguan","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangning","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2020,5,29]]},"reference":[{"key":"rl5185_bb2","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1126\/science.aad3460","volume":"351","author":"Bakelar","year":"2016","journal-title":"Science"},{"key":"rl5185_bb3","doi-asserted-by":"crossref","first-page":"535","DOI":"10.3389\/fpls.2014.00535","volume":"5","author":"Day","year":"2014","journal-title":"Front. 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