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AAC(6\u2032)-Ii enzymes target the amino group linked to the 6\u2032 C atom in an aminoglycoside. Several structures of the AAC(6\u2032)-Ii from <jats:italic>Enterococcus faecium<\/jats:italic> [Ef-AAC(6\u2032)-Ii] have been reported to date. However, the detailed mechanism of its enzymatic function remains elusive. In this study, the crystal structure of Ef-AAC(6\u2032)-Ii was determined in a novel substrate-free form. Based on structural analysis, it is proposed that Ef-AAC(6\u2032)-Ii sequentially undergoes conformational selection and induced fit for substrate binding. These results therefore provide a novel viewpoint on the mechanism of action of Ef-AAC(6\u2032)-Ii.<\/jats:p>","DOI":"10.1107\/s2053230x20009735","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T17:30:23Z","timestamp":1595957423000},"page":"364-371","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Structural analysis of a novel substrate-free form of the aminoglycoside 6\u2032-<i>N<\/i>-acetyltransferase from <i>Enterococcus faecium<\/i>"],"prefix":"10.1107","volume":"76","author":[{"given":"Hyunseok","family":"Jang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunghark","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Sook","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang Woo","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jisub","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoung Ho","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun Hyuck","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun Ho","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2020,7,28]]},"reference":[{"key":"rl5187_bb1","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1107\/S0907444912001308","volume":"68","author":"Afonine","year":"2012","journal-title":"Acta Cryst. 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