{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:45:10Z","timestamp":1791186310578,"version":"4.1.0"},"reference-count":36,"publisher":"International Union of Crystallography (IUCr)","issue":"11","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["BFU2018-101265-B-100"],"award-info":[{"award-number":["BFU2018-101265-B-100"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2020,9,7]]},"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:p>The human pathogen <jats:italic>Mycoplasma genitalium<\/jats:italic> is responsible for urethritis in men, and for cervicitis and pelvic inflammatory disease in women. The adherence of <jats:italic>M.\u00a0genitalium<\/jats:italic> to host target epithelial cells is mediated through an adhesion complex called Nap, which is essential for infectivity. Nap is a transmembrane dimer of heterodimers of the immunodominant proteins P110 and P140. The <jats:italic>M.\u00a0genitalium<\/jats:italic> genome contains multiple copies of portions that share homology with the extracellular regions of P140 and P110 encoded by the genes <jats:italic>mg191<\/jats:italic> and <jats:italic>mg192<\/jats:italic>, respectively. Homologous recombination between the genes and the copies allows the generation of a large diversity of P140 and P110 variants to overcome surveillance by the host immune system. Interestingly, the C-terminal domain (C-domain) of the extracellular region of P140, which is essential for the\u00a0function of Nap by acting as a flexible stalk anchoring the protein to the mycoplasma membrane, presents a low degree of sequence variability. In the present work, the X-ray crystal structures of two crystal forms of a construct of the P140 C-domain are reported. In both crystal forms, the construct forms a compact octamer with <jats:italic>D<\/jats:italic>4 point-group symmetry. The structure of the C-domain determined in this work presents significant differences with respect to the structure of the C-domain found recently in intact P140. The structural plasticity of the C-domain appears to be a possible mechanism that may help in the functioning of the mycoplasma adhesion complex.<\/jats:p>","DOI":"10.1107\/s2053230x20012297","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T10:07:31Z","timestamp":1602238051000},"page":"508-516","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":3,"title":["Alternative conformation of the C-domain of the P140 protein from <i>Mycoplasma genitalium<\/i>"],"prefix":"10.1107","volume":"76","author":[{"given":"David","family":"Vizarraga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"P\u00e9rez-Luque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Fita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Aparicio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2020,10,8]]},"reference":[{"key":"jb5021_bb1","doi-asserted-by":"crossref","first-page":"2877","DOI":"10.1038\/s41467-020-16511-2","volume":"11","author":"Aparicio","year":"2020","journal-title":"Nat. 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