{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:22:46Z","timestamp":1740172966217,"version":"3.37.3"},"reference-count":21,"publisher":"International Union of Crystallography (IUCr)","issue":"5","license":[{"start":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T00:00:00Z","timestamp":1619568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T00:00:00Z","timestamp":1619568000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["16H06560"],"award-info":[{"award-number":["16H06560"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004206","name":"Osaka University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004206","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003382","name":"Core Research for Evolutional Science and Technology","doi-asserted-by":"publisher","award":["JPMJCR20E1"],"award-info":[{"award-number":["JPMJCR20E1"]}],"id":[{"id":"10.13039\/501100003382","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2021,4,14]]},"published-print":{"date-parts":[[2021,5,1]]},"abstract":"<jats:p>As an essential component of protein cofactors, iron is important for all photosynthetic organisms. In <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>, iron levels are strictly controlled by proteins such as iron-assimilating protein 1 (FEA1). This periplasmic protein is expressed under conditions of iron deficiency, but its mechanisms of function remain unknown. Because FEA1 has no amino-acid similarity to protein structures in the Protein Data Bank, its crystal structure cannot be solved by molecular replacement. Here, recombinant FEA1 protein lacking the N-terminal signal sequence was successfully purified and crystals of apo FEA1 were obtained by hanging-drop vapor diffusion. Neither selenomethionine substitution nor heavy-atom derivatization was successful; therefore, the phase problem of FEA1 crystals was solved by the native sulfur SAD method using long-wavelength X-rays (2.7\u2005\u00c5). Laser-cutting technology was used to increase the signal-to-noise ratio and derive an initial structure. This study will lead to further structural studies of FEA1 to understand its function and its links to the iron-assimilation pathway.<\/jats:p>","DOI":"10.1107\/s2053230x21003952","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T09:33:13Z","timestamp":1619775193000},"page":"134-139","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystallographic analysis and phasing of iron-assimilating protein 1 (FEA1) from <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1107","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6020-9055","authenticated-orcid":true,"given":"Linda","family":"Juniar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vida","family":"Adlfar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Hippler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5354-0807","authenticated-orcid":true,"given":"Genji","family":"Kurisu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2021,4,28]]},"reference":[{"key":"nw5108_bb1","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1128\/EC.00205-07","volume":"6","author":"Allen","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"nw5108_bb2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s11120-010-9619-8","volume":"109","author":"Baba","year":"2011","journal-title":"Photosynth. Res."},{"key":"nw5108_bb3","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1107\/S2052252519002756","volume":"6","author":"Basu","year":"2019","journal-title":"IUCrJ"},{"key":"nw5108_bb4","unstructured":"Bricogne, G., Blanc, E., Brandl, M., Flensburg, C., Keller, P., Paciorek, W., Roversi, P., Sharff, A., Smart, O. S. & Vonrhein, C. (2011). BUSTER. Global Phasing Ltd, Cambridge, UK."},{"key":"nw5108_bb5","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3389\/fpls.2013.00337","volume":"4","author":"Glaesener","year":"2013","journal-title":"Front. Plant Sci."},{"key":"nw5108_bb6","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1046\/j.1469-8137.2003.00788.x","volume":"159","author":"Hanikenne","year":"2003","journal-title":"New Phytol."},{"key":"nw5108_bb7","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/290107a0","volume":"290","author":"Hendrickson","year":"1981","journal-title":"Nature"},{"key":"nw5108_bb8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"nw5108_bb9","doi-asserted-by":"crossref","first-page":"L54","DOI":"10.1143\/JJAP.44.L54","volume":"44","author":"Kitano","year":"2005","journal-title":"Jpn. J. Appl. Phys."},{"key":"nw5108_bb10","first-page":"S112","volume":"114","author":"Kobayashi","year":"1997","journal-title":"Plant Physiol."},{"key":"nw5108_bb11","doi-asserted-by":"crossref","first-page":"3160","DOI":"10.1074\/mcp.M113.028191","volume":"12","author":"Mathieu-Rivet","year":"2013","journal-title":"Mol. Cell. Proteomics"},{"key":"nw5108_bb12","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0022-2836(68)90205-2","volume":"33","author":"Matthews","year":"1968","journal-title":"J. Mol. Biol."},{"key":"nw5108_bb13","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/978-1-4939-7000-1_18","volume":"1607","author":"McCoy","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"nw5108_bb14","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/nature25982","volume":"555","author":"McQuaid","year":"2018","journal-title":"Nature"},{"key":"nw5108_bb15","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.bbamcr.2006.04.007","volume":"1763","author":"Merchant","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"nw5108_bb16","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1107\/S2052252515008337","volume":"2","author":"Rose","year":"2015","journal-title":"IUCrJ"},{"key":"nw5108_bb17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00425-001-0711-3","volume":"215","author":"Rubinelli","year":"2002","journal-title":"Planta"},{"key":"nw5108_bb18","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1093\/oxfordjournals.pcp.a029383","volume":"39","author":"Sasaki","year":"1998","journal-title":"Plant Cell Physiol."},{"key":"nw5108_bb19","doi-asserted-by":"crossref","first-page":"3921","DOI":"10.1105\/tpc.112.102491","volume":"24","author":"Urzica","year":"2012","journal-title":"Plant Cell"},{"key":"nw5108_bb20","first-page":"215","volume":"364","author":"Vonrhein","year":"2007","journal-title":"Methods Mol. Biol."},{"key":"nw5108_bb21","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Cryst. D"}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2021\/05\/00\/nw5108\/nw5108.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2021\/05\/00\/nw5108\/nw5108.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T10:38:30Z","timestamp":1736764710000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X21003952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,28]]},"references-count":21,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["S2053230X21003952"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x21003952","relation":{},"ISSN":["2053-230X"],"issn-type":[{"type":"electronic","value":"2053-230X"}],"subject":[],"published":{"date-parts":[[2021,4,28]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?nw5108","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"15 March 2021","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"14 April 2021","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"28 April 2021","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"\u00a9 2021 Juniar et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}