{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:22:49Z","timestamp":1740172969644,"version":"3.37.3"},"reference-count":33,"publisher":"International Union of Crystallography (IUCr)","issue":"12","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2020YFC1807000"],"award-info":[{"award-number":["2020YFC1807000"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870053","31800627"],"award-info":[{"award-number":["31870053","31800627"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2021,10,21]]},"published-print":{"date-parts":[[2021,12,1]]},"abstract":"<jats:p>Oligoribonuclease (Orn), a member of the DEDDh superfamily, can hydrolyse 2\u20135\u2005nt nanoRNAs to mononucleotides. It is involved in maintaining the intracellular levels of RNA, c-di-GMP signalling and transcription initiation in many bacterial species. Here, the crystal structure of Orn from <jats:italic>Vibrio cholerae<\/jats:italic> O1 El Tor (<jats:italic>Vc<\/jats:italic>Orn) is reported at a resolution of 1.7\u2005\u00c5. <jats:italic>Vc<\/jats:italic>Orn, which consists of\u00a0nine \u03b1-helices and six \u03b2-strands, crystallizes with a single monomer in the asymmetric unit but forms a homodimer via crystallographic twofold symmetry. Electron density is observed in the active pocket that corresponds to an intersubunit N-terminal expression tag with sequence GPLGSHHH. The positively charged N-terminal tag binds in the negatively charged nucleotide-binding pocket with a buried surface area of \u223c500\u2005\u00c5<jats:sup>2<\/jats:sup>. The N-terminal tag interacts with <jats:italic>Vc<\/jats:italic>Orn via \u03c0\u2013\u03c0 stacking with two conserved residues involved in nucleotide binding, as well as via salt bridges and hydrogen bonds. The structure reported here reveals that the active pocket can accommodate polypeptides in addition to nucleotides, thus providing an important starting point for investigation into substrate modification and inhibitor design targeting <jats:italic>Vc<\/jats:italic>Orn.<\/jats:p>","DOI":"10.1107\/s2053230x21011043","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T16:24:05Z","timestamp":1636647845000},"page":"437-443","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystal structure of oligoribonuclease from <i>Vibrio cholerae<\/i> O1 El Tor with bound peptide"],"prefix":"10.1107","volume":"77","author":[{"given":"Jianyu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyuan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1016-1874","authenticated-orcid":true,"given":"Qionglin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5173-5080","authenticated-orcid":true,"given":"Mark","family":"Bartlam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"key":"tb5170_bb2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.micres.2016.05.002","volume":"188-189","author":"Chen","year":"2016","journal-title":"Microbiol. 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