{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:22:32Z","timestamp":1740172952475,"version":"3.37.3"},"reference-count":26,"publisher":"International Union of Crystallography (IUCr)","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100004012","name":"Jane ja Aatos Erkon S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004012","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004155","name":"Magnus Ehrnroothin S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004155","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2021,12,3]]},"published-print":{"date-parts":[[2022,1,1]]},"abstract":"<jats:p>Synaptic adhesion molecules are major organizers of the neuronal network and play a crucial role in the regulation of synapse development and maintenance in the brain. Synaptic adhesion-like molecules (SALMs) and leukocyte common antigen-related receptor protein tyrosine phosphatases (LAR-PTPs) are adhesion protein families with established synaptic function. Dysfunction of several synaptic adhesion molecules has been linked to cognitive disorders such as autism spectrum disorders and schizophrenia. A recent study of the binding and complex structure of SALM3 and PTP\u03c3 using small-angle X-ray scattering revealed a 2:2 complex similar to that observed for the interaction of human SALM5 and PTP\u03b4. However, the molecular structure of the SALM3\u2013PTP\u03c3 complex remains to be determined beyond the small-angle X-ray scattering model. Here, the expression, purification, crystallization and initial 6.5\u2005\u00c5 resolution structure of the mouse SALM3\u2013PTP\u03c3 complex are reported, which further verifies the formation of a 2:2 trans-heterotetrameric complex similar to the crystal structure of human SALM5\u2013PTP\u03b4 and validates the architecture of the previously reported small-angle scattering-based solution structure of the SALM3\u2013PTP\u03c3 complex. Details of the protein expression and purification, crystal optimization trials, and the initial structure solution and data analysis are provided.<\/jats:p>","DOI":"10.1107\/s2053230x21012905","type":"journal-article","created":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T11:52:53Z","timestamp":1640173973000},"page":"39-44","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystallization and low-resolution structure solution of the SALM3\u2013PTP\u03c3 synaptic adhesion complex"],"prefix":"10.1107","volume":"78","author":[{"given":"Sudeep","family":"Karki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5094-227X","authenticated-orcid":true,"given":"Tommi","family":"Kajander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2022,1,1]]},"reference":[{"key":"nj5312_bb1","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1093\/nar\/16.3.1043","volume":"16","author":"Bunch","year":"1988","journal-title":"Nucleic Acids Res."},{"key":"nj5312_bb2","doi-asserted-by":"crossref","first-page":"26676","DOI":"10.1038\/srep26676","volume":"6","author":"Choi","year":"2016","journal-title":"Sci. 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