{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T23:29:25Z","timestamp":1777678165806,"version":"3.51.4"},"reference-count":40,"publisher":"International Union of Crystallography (IUCr)","issue":"2","license":[{"start":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T00:00:00Z","timestamp":1643328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T00:00:00Z","timestamp":1643328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institutes of Health, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201700059C"],"award-info":[{"award-number":["HHSN272201700059C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institutes of Health, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201200025C"],"award-info":[{"award-number":["HHSN272201200025C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institutes of Health, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272200700057C"],"award-info":[{"award-number":["HHSN272200700057C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1U01GM138433-01"],"award-info":[{"award-number":["1U01GM138433-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2021,12,23]]},"published-print":{"date-parts":[[2022,2,1]]},"abstract":"<jats:p>Giardiasis is the most prevalent diarrheal disease globally and affects humans and animals. It is a significant problem in developing countries, the number one cause of travelers' diarrhea and affects children and immunocompromised individuals, especially HIV-infected individuals. Giardiasis is treated with antibiotics (tinidazole and metronidazole) that are also used for other infections such as trichomoniasis. The ongoing search for new therapeutics for giardiasis includes characterizing the structure and function of proteins from the causative protozoan <jats:italic>Giardia lamblia<\/jats:italic>. These proteins include hypothetical proteins that share 30% sequence identity or less with proteins of known structure. Here, the atomic resolution structure of a 15.6\u2005kDa protein was determined by molecular replacement. The structure has the two-layer \u03b1\u03b2-sandwich topology observed in the prototypical endoribonucleases L-PSPs (liver perchloric acid-soluble proteins) with conserved allosteric active sites containing small molecules from the crystallization solution. This article is an educational collaboration between Hampton University and the Seattle Structural Genomics Center for Infectious Disease.<\/jats:p>","DOI":"10.1107\/s2053230x21013595","type":"journal-article","created":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T09:23:48Z","timestamp":1643361828000},"page":"59-65","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Crystal structure of a hypothetical protein from <i>Giardia lamblia<\/i>"],"prefix":"10.1107","volume":"78","author":[{"given":"Dylan K.","family":"Beard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seonna","family":"Bristol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla","family":"Cosby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amber","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Courtney","family":"Manning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lionel","family":"Perry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren","family":"Snapp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3796-3102","authenticated-orcid":true,"given":"Arian","family":"Toy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla","family":"Wheeler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart","family":"Staker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy L.","family":"Arakaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-3243","authenticated-orcid":true,"given":"Jan","family":"Abendroth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandhya","family":"Subrahamanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0474-8003","authenticated-orcid":true,"given":"Thomas E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-0513","authenticated-orcid":true,"given":"Peter J.","family":"Myler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4043-2700","authenticated-orcid":true,"given":"Oluwatoyin A.","family":"Asojo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2022,1,28]]},"reference":[{"key":"ft5116_bb1","doi-asserted-by":"crossref","first-page":"6069","DOI":"10.1093\/nar\/18.20.6069","volume":"18","author":"Aslanidis","year":"1990","journal-title":"Nucleic Acids Res."},{"key":"ft5116_bb2","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1107\/S1744309111018367","volume":"67","author":"Bryan","year":"2011","journal-title":"Acta Cryst. F"},{"key":"ft5116_bb3","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1107\/S0907444903007017","volume":"59","author":"Burman","year":"2003","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb4","doi-asserted-by":"crossref","unstructured":"Burman, J. D., Stevenson, C. E. M., Sawers, R. G. & Lawson, D. M. (2007). BMC Struct. Biol. 7, 30.","DOI":"10.1186\/1472-6807-7-30"},{"key":"ft5116_bb5","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1107\/S1744309111017374","volume":"67","author":"Choi","year":"2011","journal-title":"Acta Cryst. F"},{"key":"ft5116_bb6","doi-asserted-by":"crossref","first-page":"596","DOI":"10.4269\/ajtmh.15-0111","volume":"93","author":"Daniels","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ft5116_bb7","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1002\/prot.10151","volume":"48","author":"Deaconescu","year":"2002","journal-title":"Proteins"},{"key":"ft5116_bb8","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb9","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb10","doi-asserted-by":"crossref","first-page":"76","DOI":"10.3855\/jidc.5785","volume":"9","author":"Escobedo","year":"2015","journal-title":"J. Infect. Dev. Ctries"},{"key":"ft5116_bb11","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1093\/bbb\/zbaa037","volume":"85","author":"Fujimoto","year":"2021","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ft5116_bb12","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1093\/bioinformatics\/15.4.305","volume":"15","author":"Gouet","year":"1999","journal-title":"Bioinformatics"},{"key":"ft5116_bb13","doi-asserted-by":"crossref","first-page":"3320","DOI":"10.1093\/nar\/gkg556","volume":"31","author":"Gouet","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"ft5116_bb14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s10969-008-9045-8","volume":"10","author":"Headd","year":"2009","journal-title":"J. Struct. Funct. Genomics"},{"key":"ft5116_bb15","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/pro.3749","volume":"29","author":"Holm","year":"2020","journal-title":"Protein Sci."},{"key":"ft5116_bb16","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.gtc.2006.03.006","volume":"35","author":"Huang","year":"2006","journal-title":"Gastroenterol. Clin. North Am."},{"key":"ft5116_bb99","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb17","doi-asserted-by":"crossref","first-page":"BSR20180768","DOI":"10.1042\/BSR20180768","volume":"38","author":"Kim","year":"2018","journal-title":"Biosci. Rep."},{"key":"ft5116_bb18","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1046\/j.1365-2443.2001.00443.x","volume":"6","author":"Kim","year":"2001","journal-title":"Genes Cells"},{"key":"ft5116_bb19","doi-asserted-by":"crossref","first-page":"4089","DOI":"10.1128\/JB.00201-10","volume":"192","author":"Kim","year":"2010","journal-title":"J. Bacteriol."},{"key":"ft5116_bb20","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.1107\/S0907444904026460","volume":"60","author":"Krissinel","year":"2004","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb21","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.bbrc.2019.11.139","volume":"522","author":"Kwon","year":"2020","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ft5116_bb22","doi-asserted-by":"crossref","first-page":"3454","DOI":"10.1074\/jbc.M111.304477","volume":"287","author":"Lambrecht","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ft5116_bb23","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1107\/S0907444907049839","volume":"64","author":"Lebedev","year":"2008","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb24","first-page":"177","volume":"142","author":"Lobovsk\u00e1","year":"2003","journal-title":"Cas. Lek. Cesk."},{"key":"ft5116_bb25","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.prevetmed.2013.07.002","volume":"116","author":"McIntyre","year":"2014","journal-title":"Prev. Vet. Med."},{"key":"ft5116_bb26","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1021\/bc0341066","volume":"14","author":"Mistiniene","year":"2003","journal-title":"Bioconjug. Chem."},{"key":"ft5116_bb27","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/prot.20778","volume":"62","author":"Miyakawa","year":"2006","journal-title":"Proteins"},{"key":"ft5116_bb28","doi-asserted-by":"crossref","first-page":"20688","DOI":"10.1074\/jbc.274.29.20688","volume":"274","author":"Morishita","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ft5116_bb29","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1107\/S0907444911001314","volume":"67","author":"Murshudov","year":"2011","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb30","doi-asserted-by":"crossref","unstructured":"Niehaus, T. D., Gerdes, S., Hodge-Hanson, K., Zhukov, A., Cooper, A. J., ElBadawi-Sidhu, M., Fiehn, O., Downs, D. M. & Hanson, A. D. (2015). BMC Genomics, 16, 382.","DOI":"10.1186\/s12864-015-1584-3"},{"key":"ft5116_bb31","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.jsb.2011.05.005","volume":"175","author":"Pu","year":"2011","journal-title":"J. Struct. Biol."},{"key":"ft5116_bb32","doi-asserted-by":"crossref","first-page":"W320","DOI":"10.1093\/nar\/gku316","volume":"42","author":"Robert","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ft5116_bb33","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1107\/S2053230X15004677","volume":"71","author":"Serbzhinskiy","year":"2015","journal-title":"Acta Cryst. F"},{"key":"ft5116_bb34","doi-asserted-by":"crossref","first-page":"13074","DOI":"10.1073\/pnas.96.23.13074","volume":"96","author":"Sinha","year":"1999","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"ft5116_bb35","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pep.2005.01.016","volume":"41","author":"Studier","year":"2005","journal-title":"Protein Expr. Purif."},{"key":"ft5116_bb36","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.ijpara.2013.06.007","volume":"43","author":"Thompson","year":"2013","journal-title":"Int. J. Parasitol."},{"key":"ft5116_bb37","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1107\/S0907444909042589","volume":"66","author":"Vagin","year":"2010","journal-title":"Acta Cryst. D"},{"key":"ft5116_bb38","doi-asserted-by":"crossref","first-page":"2428","DOI":"10.1110\/ps.8.11.2428","volume":"8","author":"Volz","year":"1999","journal-title":"Protein Sci."},{"key":"ft5116_bb39","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.bbrc.2010.05.071","volume":"397","author":"Zhang","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."}],"updated-by":[{"DOI":"10.1107\/s2053230x22001704","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000}}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2022\/02\/00\/ft5116\/ft5116.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2022\/02\/00\/ft5116\/ft5116.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T10:49:45Z","timestamp":1736765385000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X21013595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,28]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["S2053230X21013595"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x21013595","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1107\/S2053230X21013595\/ft5116sup1.pdf","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb3i3f\/pdb","asserted-by":"subject"}],"correction":[{"id-type":"doi","id":"10.1107\/S2053230X22001704","asserted-by":"object"}]},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,28]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?ft5116","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"13 October 2021","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"23 December 2021","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"28 January 2022","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?ft5116"}},{"value":"\u00a9 2022 Dylan Beard et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}