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There is a need to develop new therapeutics due to drug resistance and the failure of current treatments to clear persistent infections. Structures of potential <jats:italic>C. trachomatis<\/jats:italic> rational drug-discovery targets, including <jats:italic>C. trachomatis<\/jats:italic> inorganic pyrophosphatase (<jats:italic>Ct<\/jats:italic>PPase), have been determined by\u00a0the Seattle Structural Genomics Center for Infectious Disease. Inorganic pyrophosphatase hydrolyzes inorganic pyrophosphate during metabolism. Furthermore, bacterial inorganic pyrophosphatases have shown promise for therapeutic discovery. Here, a 2.2\u2005\u00c5 resolution X-ray structure of <jats:italic>Ct<\/jats:italic>PPase is reported. The crystal structure of <jats:italic>Ct<\/jats:italic>PPase reveals shared structural features that may facilitate the repurposing of inhibitors identified for bacterial inorganic pyrophosphatases as starting points for new therapeutics for <jats:italic>C. trachomatis.<\/jats:italic>\n               <\/jats:p>","DOI":"10.1107\/s2053230x22002138","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T12:09:00Z","timestamp":1646050140000},"page":"135-142","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Crystal structure of an inorganic pyrophosphatase from <i>Chlamydia trachomatis<\/i> D\/UW-3\/Cx"],"prefix":"10.1107","volume":"78","author":[{"given":"Jasmine","family":"Maddy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart L.","family":"Staker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandhya","family":"Subramanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-3243","authenticated-orcid":true,"given":"Jan","family":"Abendroth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0474-8003","authenticated-orcid":true,"given":"Thomas E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-0513","authenticated-orcid":true,"given":"Peter J.","family":"Myler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Hybiske","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4043-2700","authenticated-orcid":true,"given":"Oluwatoyin A.","family":"Asojo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"329","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"key":"tb5175_bb2","doi-asserted-by":"crossref","first-page":"6069","DOI":"10.1093\/nar\/18.20.6069","volume":"18","author":"Aslanidis","year":"1990","journal-title":"Nucleic Acids Res."},{"key":"tb5175_bb3","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1107\/S1744309113027371","volume":"69","author":"Barta","year":"2013","journal-title":"Acta Cryst. 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