{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:15:20Z","timestamp":1790734520765,"version":"4.1.0"},"reference-count":12,"publisher":"International Union of Crystallography (IUCr)","issue":"7","license":[{"start":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T00:00:00Z","timestamp":1656892800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T00:00:00Z","timestamp":1656892800000},"content-version":"tdm","delay-in-days":3,"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100002784","name":"Canada Excellence Research Chairs, Government of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002784","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP 48370"],"award-info":[{"award-number":["MOP 48370"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2022,6,24]]},"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:p>\n                    The bacterium\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    is a causative agent of Legionnaires' disease. It utilizes the Dot\/Icm type IV secretion system (T4SS) to deliver over 300 effector proteins into the host cell, leading to modification of cellular processes and creating a safe environment for bacterial proliferation. Dot\/Icm is a multi-subunit molecular machine. The effectors are recognized by the inner membrane-embedded coupling complex (T4CC), which then delivers them to the translocation apparatus. This T4CC subcomplex is made up of DotL, DotM, DotN, IcmS, IcmW, LvgA, DotY and DotZ, and its structure was recently determined by cryo-EM. DotY is a highly mobile component of this subcomplex and its structure was only partially defined. DotY is a unique component of the T4SS that is only found in the\n                    <jats:italic>Legionella<\/jats:italic>\n                    genus. Here, the crystal structure of DotY on its own is presented and its fold and the connectivity of its secondary-structure elements are established. The protein is divided into three segments. The first and last segments form a four-helix bundle domain, while the middle segment forms an \u03b1\/\u03b2 domain that has a unique fold. The flexibility of the interdomain linkers allows the reorientation of the two domains between that observed in the crystal structure and that assumed within the T4CC subcomplex.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x22006604","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T06:15:12Z","timestamp":1656915312000},"page":"276-280","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Structural study of\n                    <i>Legionella pneumophila<\/i>\n                    effector DotY (Lpg0294), a component of the Dot\/Icm type IV secretion system"],"prefix":"10.1107","volume":"78","author":[{"given":"Ivy Yeuk Wah","family":"Chung","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/010x8gc63","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biochemistry, Microbiology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4579-1881","authenticated-orcid":true,"given":"Miroslaw","family":"Cygler","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/010x8gc63","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biochemistry, Microbiology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2022,7,4]]},"reference":[{"key":"pg5091_bb2","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"pg5091_bb3","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-1-59745-196-3_7","volume":"498","author":"Eschenfeldt","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"pg5091_bb4","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1107\/S1600577514005578","volume":"21","author":"Fodje","year":"2014","journal-title":"J. Synchrotron Rad."},{"key":"pg5091_bb5","doi-asserted-by":"crossref","unstructured":"Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M. R., Appel, R. D. & Bairoch, A. (2005). The Proteomics Protocols Handbook, edited by J. M. Walker, pp. 571-607. Totowa: Humana Press.","DOI":"10.1385\/1-59259-890-0:571"},{"key":"pg5091_bb6","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1111\/febs.15794","volume":"289","author":"Grishin","year":"2022","journal-title":"FEBS J."},{"key":"pg5091_bb7","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"pg5091_bb8","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/1348-0421.12951","volume":"66","author":"Kitao","year":"2022","journal-title":"Microbiol. Immunol."},{"key":"pg5091_bb1","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","volume":"75","author":"Liebschner","year":"2019","journal-title":"Acta Cryst. D"},{"key":"pg5091_bb9","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/mmi.14847","volume":"117","author":"Mac\u00e9","year":"2022","journal-title":"Mol. Microbiol."},{"key":"pg5091_bb10","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1038\/s41467-020-16681-z","volume":"11","author":"Meir","year":"2020","journal-title":"Nat. Commun."},{"key":"pg5091_bb11","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.mib.2014.11.008","volume":"23","author":"Prashar","year":"2015","journal-title":"Curr. Opin. Microbiol."},{"key":"pg5091_bb12","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1126\/science.1179513","volume":"327","author":"Tanaka","year":"2010","journal-title":"Science"}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2022\/07\/00\/pg5091\/pg5091.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/f\/issues\/2022\/07\/00\/pg5091\/pg5091.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T06:10:38Z","timestamp":1736748638000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X22006604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,1]]},"references-count":12,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["S2053230X22006604"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x22006604","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.2210\/pdb7ups\/pdb","asserted-by":"subject"}]},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,1]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?pg5091","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"10 May 2022","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"24 June 2022","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"4 July 2022","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?pg5091"}},{"value":"\u00a9 Chung and Cygler","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}},{"value":"IUCr licence to publish","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/journals.iucr.org\/copyright\/licencetopublish.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}