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Previously, 5\u2032-\n                    <jats:italic>O<\/jats:italic>\n                    -[\n                    <jats:italic>N<\/jats:italic>\n                    -(11-phenoxyundecanoyl)sulfamoyl]adenosine (PhU-AMS) has been shown to be a general inhibitor of fatty-acid-adenylating enzymes (FadDs) in\n                    <jats:italic>Mtb<\/jats:italic>\n                    . However, the fatty acyl-AMP ligase (FAAL) class of FadDs, which includes\n                    <jats:italic>Mtb<\/jats:italic>\n                    FadD23, appears to be functionally nonredundant in the production of multiple fatty acids. In this study, the ability of PhU-AMS to bind to\n                    <jats:italic>Mtb<\/jats:italic>\n                    FadD23 was examined under\n                    <jats:italic>in vitro<\/jats:italic>\n                    conditions. The crystal structure of the\n                    <jats:italic>Mtb<\/jats:italic>\n                    FadD23\u2013PhU-AMS complex was determined at a resolution of 2.64\u2005\u00c5. Novel features were identified by structural analysis and comparison. Although PhU-AMS could bind to\n                    <jats:italic>Mtb<\/jats:italic>\n                    FadD23, it did not inhibit the FAAL adenylation activity of\n                    <jats:italic>Mtb<\/jats:italic>\n                    FadD23. However, PhU-AMS improved the main\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    value in a differential scanning fluorimetry assay, and a structural comparison of\n                    <jats:italic>Mtb<\/jats:italic>\n                    FadD23\u2013PhU-AMS with FadD32 and PA1221 suggested that PhU-AMS blocks the loading of the acyl chain onto Pks2. This study sheds light on the structure-based design of specific inhibitors of\n                    <jats:italic>Mtb<\/jats:italic>\n                    FadD23 and general inhibitors of FAALs.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x23005836","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T12:41:32Z","timestamp":1689684092000},"page":"208-216","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Structural basis for the development of potential inhibitors targeting FadD23 from\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1107","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0093-2775","authenticated-orcid":true,"given":"Mengrong","family":"Yan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7651-2304","authenticated-orcid":true,"given":"Mengyuan","family":"Ma","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9796-2641","authenticated-orcid":true,"given":"Yangzi","family":"Cao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"department":["College of Pharmacy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8693-6747","authenticated-orcid":true,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"department":["State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for Cell Responses, College of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2023,7,31]]},"reference":[{"key":"no5201_bb2","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1038\/nchembio.143","volume":"5","author":"Arora","year":"2009","journal-title":"Nat. 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