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The intracellular concentration of PP\n                    <jats:sub>i<\/jats:sub>\n                    must be regulated as buildup can inhibit many critical cellular processes. Inorganic pyrophosphatases (PPases) hydrolyze PP\n                    <jats:sub>i<\/jats:sub>\n                    into two orthophosphates (P\n                    <jats:sub>i<\/jats:sub>\n                    ), preventing the toxic accumulation of the PP\n                    <jats:sub>i<\/jats:sub>\n                    byproduct in cells and making P\n                    <jats:sub>i<\/jats:sub>\n                    available for use in biosynthetic pathways. Here, the crystal structure of a family I inorganic pyrophosphatase from\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    is reported at 2.0\u2005\u00c5 resolution.\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    PPase (LpPPase) adopts a homohexameric assembly and shares the oligonucleotide\/oligosaccharide-binding (OB) \u03b2-barrel core fold common to many other bacterial family I PPases. LpPPase demonstrated hydrolytic activity against a general substrate, with Mg\n                    <jats:sup>2+<\/jats:sup>\n                    being the preferred metal cofactor for catalysis. Legionnaires' disease is a severe respiratory infection caused primarily by\n                    <jats:italic>L.\u00a0pneumophila<\/jats:italic>\n                    , and thus increased characterization of the\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    proteome is of interest.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x23008002","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T07:03:13Z","timestamp":1695020593000},"page":"257-266","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of a family I inorganic pyrophosphatase from\n                    <i>Legionella pneumophila<\/i>\n                    Philadelphia 1"],"prefix":"10.1107","volume":"79","author":[{"given":"Julia","family":"Moorefield","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1971-6706","authenticated-orcid":true,"given":"Yagmur","family":"Konuk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan O.","family":"Norman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-3243","authenticated-orcid":true,"given":"Jan","family":"Abendroth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0474-8003","authenticated-orcid":true,"given":"Thomas E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald D.","family":"Lorimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Mayclin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart L.","family":"Staker","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cvxb145","id-type":"ROR","asserted-by":"publisher"}],"department":["Seattle Children's Research Institute"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin K.","family":"Craig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayleigh F.","family":"Barett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn K.","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley C.","family":"Van Voorhis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-0513","authenticated-orcid":true,"given":"Peter J.","family":"Myler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystle J.","family":"McLaughlin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"nj5322_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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