{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:13:59Z","timestamp":1785413639957,"version":"3.56.0"},"reference-count":35,"publisher":"International Union of Crystallography (IUCr)","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100017210","name":"Centre National de la Recherche Scientifique, Institut des Sciences Biologiques","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017210","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2023,12,13]]},"published-print":{"date-parts":[[2024,1,1]]},"abstract":"<jats:p>\n                    <jats:italic>Nocardia<\/jats:italic>\n                    are Gram-positive bacteria from the Actinobacteria phylum. Some\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species can infect humans and are usually considered to be opportunist pathogens, as they often infect immunocompromised patients. Although their clinical incidence is low, many\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species are now considered to be emerging pathogens. Primary sites of infection by\n                    <jats:italic>Nocardia<\/jats:italic>\n                    are the skin or the lungs, but dissemination to other body parts is very frequent. These disseminated infections are very difficult to treat and thus are tackled with multiple classes of antibiotics, in addition to the traditional treatment targeting the folate pathway. \u03b2-Lactams are often included in the regimen, but many\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species present moderate or strong resistance to some members of this drug class. Genomic, microbiological and biochemical studies have reported the presence of class A \u03b2-lactamases (ABLs) in a handful of\n                    <jats:italic>Nocardia<\/jats:italic>\n                    species, but no structural investigation of\n                    <jats:italic>Nocardia<\/jats:italic>\n                    \u03b2-lactamases has yet been performed. In this study, the expression, purification and preliminary biochemical characterization of an ABL from an\n                    <jats:italic>N. cyriacigeorgica<\/jats:italic>\n                    (NCY-1) clinical strain are reported. The crystallization and the very high resolution crystal structure of NCY-1 are also described. The sequence and structural analysis of the protein demonstrate that NCY-1 belongs to the class A1 \u03b2-lactamases and show its very high conservation with ABLs from other human-pathogenic\n                    <jats:italic>Nocardia<\/jats:italic>\n                    . In addition, the presence of one molecule of citrate tightly bound in the catalytic site of the enzyme is described. This structure may provide a solid basis for future drug development to specifically target\n                    <jats:italic>Nocardia<\/jats:italic>\n                    spp. \u03b2-lactamases.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x23010671","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T11:44:30Z","timestamp":1703159070000},"page":"13-21","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Biochemical and structural characterization of a class A \u03b2-lactamase from\n                    <i>Nocardia cyriacigeorgica<\/i>"],"prefix":"10.1107","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5362-6099","authenticated-orcid":true,"given":"J\u00e9r\u00f4me","family":"Feuillard","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051escj72","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1880-8175","authenticated-orcid":true,"given":"Julie","family":"Couston","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051escj72","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3944-3820","authenticated-orcid":true,"given":"Yvonne","family":"Benito","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01502ca60","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5144-1987","authenticated-orcid":true,"given":"Elisabeth","family":"Hodille","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01502ca60","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8193-1437","authenticated-orcid":true,"given":"Oana","family":"Dumitrescu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01502ca60","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7860-3464","authenticated-orcid":true,"given":"Micka\u00ebl","family":"Blaise","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051escj72","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,1,1]]},"reference":[{"key":"ow5037_bb1","doi-asserted-by":"crossref","first-page":"W530","DOI":"10.1093\/nar\/gkab294","volume":"49","author":"Adasme","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ow5037_bb2","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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