{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:11:40Z","timestamp":1785427900658,"version":"3.56.0"},"reference-count":21,"publisher":"International Union of Crystallography (IUCr)","issue":"7","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1F1A1047349"],"award-info":[{"award-number":["2021R1F1A1047349"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009819","name":"Busan Metropolitan City","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009819","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Busan Techno Park"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2024,6,15]]},"published-print":{"date-parts":[[2024,7,1]]},"abstract":"<jats:p>\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    can reside and persist in deep tissues; latent tuberculosis can evade immune detection and has a unique mechanism to convert it into active disease through reactivation.\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    Rv1421 (MtRv1421) is a hypothetical protein that has been proposed to be involved in\u00a0nucleotide binding-related metabolism in cell-growth and cell-division processes. However, due to a lack of structural information, the detailed function of MtRv1421 remains unclear. In this study, a truncated N-terminal domain (NTD) of MtRv1421, which contains a Walker A\/B-like motif, was purified and crystallized using PEG 400 as a precipitant. The crystal of MtRv1421-NTD diffracted to a resolution of 1.7\u2005\u00c5 and was considered to belong to either the\n                    <jats:italic>C<\/jats:italic>\n                    -centered monoclinic space group\n                    <jats:italic>C<\/jats:italic>\n                    2 or the\n                    <jats:italic>I<\/jats:italic>\n                    -centered orthorhombic space group\n                    <jats:italic>I<\/jats:italic>\n                    222, with unit-cell parameters\n                    <jats:italic>a<\/jats:italic>\n                    = 124.01,\n                    <jats:italic>b<\/jats:italic>\n                    = 58.55,\n                    <jats:italic>c<\/jats:italic>\n                    = 84.87\u2005\u00c5, \u03b2\u00a0=\u00a0133.12\u00b0 or\n                    <jats:italic>a<\/jats:italic>\n                    = 58.53,\n                    <jats:italic>b<\/jats:italic>\n                    = 84.86,\n                    <jats:italic>c<\/jats:italic>\n                    = 90.52\u2005\u00c5, respectively. The asymmetric units of the\n                    <jats:italic>C<\/jats:italic>\n                    2 or\n                    <jats:italic>I<\/jats:italic>\n                    222 crystals contained two or one monomers, respectively. In\u00a0terms of the binding ability of MtRv1421-NTD to various ligands, uridine diphosphate (UDP) and UDP-\n                    <jats:italic>N<\/jats:italic>\n                    -acetylglucosamine significantly increased the melting temperature of MtRv1421-NTD, which indicates structural stabilization through the binding of these ligands. Altogether, the results reveal that a UDP moiety may be required for the interaction of MtRv1421-NTD as a nucleotide-binding protein with its ligand.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x24005831","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T06:45:12Z","timestamp":1718779512000},"page":"135-141","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Preliminary X-ray diffraction and ligand-binding analyses of the N-terminal domain of hypothetical protein Rv1421 from\n                    <i>Mycobacterium tuberculosis<\/i>\n                    H37Rv"],"prefix":"10.1107","volume":"80","author":[{"given":"Jihyun","family":"Park","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tawch75","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Clinical Laboratory Science, College of Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Jeong","family":"Cheon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tawch75","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Clinical Laboratory Science, College of Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6145-3756","authenticated-orcid":true,"given":"Yoon Chae","family":"Jeong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tawch75","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Clinical Laboratory Science, College of Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Seog","family":"Lee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tawch75","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Clinical Laboratory Science, College of Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"ek5038_bb1","doi-asserted-by":"crossref","first-page":"e20","DOI":"10.1016\/S2666-5247(22)00359-7","volume":"4","author":"Bagcchi","year":"2023","journal-title":"Lancet Microbe"},{"key":"ek5038_bb2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/msb.2008.63","volume":"4","author":"Bal\u00e1zsi","year":"2008","journal-title":"Mol. 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