{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:52:52Z","timestamp":1785405172914,"version":"3.56.0"},"reference-count":0,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["75N93022C00036"],"award-info":[{"award-number":["75N93022C00036"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U01GM138433"],"award-info":[{"award-number":["U01GM138433"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2024,7,17]]},"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:p>\n                    <jats:italic>Brucella ovis<\/jats:italic>\n                    is an etiologic agent of ovine epididymitis and brucellosis that causes global devastation in sheep, rams, goats, small ruminants and deer. There are no cost-effective methods for the worldwide eradication of ovine brucellosis.\n                    <jats:italic>B. ovis<\/jats:italic>\n                    and other protein targets from various\n                    <jats:italic>Brucella<\/jats:italic>\n                    species are currently in the pipeline for high-throughput structural analysis at the Seattle Structural Genomics Center for Infectious Disease (SSGCID), with the aim of identifying new therapeutic targets. Furthermore, the wealth of structures generated are effective tools for teaching scientific communication, structural science and biochemistry. One of these structures,\n                    <jats:italic>B. ovis<\/jats:italic>\n                    leucine-, isoleucine-, valine-, threonine- and alanine-binding protein (\n                    <jats:italic>Bo<\/jats:italic>\n                    LBP), is a putative periplasmic amino acid-binding protein.\n                    <jats:italic>Bo<\/jats:italic>\n                    LBP shares less than 29% sequence identity with any other structure in the Protein Data Bank. The production, crystallization and high-resolution structures of\n                    <jats:italic>Bo<\/jats:italic>\n                    LBP are reported.\n                    <jats:italic>Bo<\/jats:italic>\n                    LBP is a prototypical bacterial periplasmic amino acid-binding protein with the characteristic Venus flytrap topology of two globular domains encapsulating a large central cavity containing the peptide-binding region. The central cavity contains small molecules usurped from the crystallization milieu. The reported structures reveal the conformational flexibility of the central cavity in the absence of bound peptides. The structural similarity to other LBPs can be exploited to accelerate drug repurposing.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x24007027","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T07:05:56Z","timestamp":1721631956000},"page":"193-199","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":2,"title":["Structures of\n                    <i>Brucella ovis<\/i>\n                    leucine-, isoleucine-, valine-, threonine- and alanine-binding protein reveal a conformationally flexible peptide-binding cavity"],"prefix":"10.1107","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4539-3971","authenticated-orcid":true,"given":"Graham","family":"Chakafana","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05fde5z47","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry and 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Communications"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2024\/09\/00\/ir5032\/ir5032.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2024\/09\/00\/ir5032\/ir5032.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T09:41:49Z","timestamp":1725529309000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X24007027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,23]]},"references-count":0,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["S2053230X24007027"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x24007027","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1107\/S2053230X24007027\/ir5032sup1.pdf","asserted-by":"subject"},{"id-type":"doi","id":"10.1107\/S2053230X24007027\/ir5032sup2.mov","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb4xfk\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb7jfn\/pdb","asserted-by":"subject"}]},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,23]]},"assertion":[{"value":"Acta 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