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Primaquine, the preferred treatment for\n                    <jats:italic>P. vivax<\/jats:italic>\n                    malaria, is contraindicated in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency, a common genetic cause of hemolytic anemia, that affects \u223c2.5% of the world's population and \u223c8% of the population in areas of the world where\n                    <jats:italic>P. vivax<\/jats:italic>\n                    malaria is endemic. The Seattle Structural Genomics Center for Infectious Disease (SSGCID) conducted a structure\u2013function analysis of\n                    <jats:italic>P.\u00a0vivax<\/jats:italic>\n                    <jats:italic>N<\/jats:italic>\n                    -myristoyltransferase (\n                    <jats:italic>Pv<\/jats:italic>\n                    NMT) as part of efforts to develop alternative malaria drugs.\n                    <jats:italic>Pv<\/jats:italic>\n                    NMT catalyzes the attachment of myristate to the N-terminal glycine of many proteins, and this critical post-translational modification is required for the survival of\n                    <jats:italic>P. vivax<\/jats:italic>\n                    . The first step is the formation of a\u00a0\n                    <jats:italic>Pv<\/jats:italic>\n                    NMT\u2013myristoyl\u2013CoA binary complex that can bind to peptides. Understanding how inhibitors prevent protein binding will facilitate the development of\n                    <jats:italic>Pv<\/jats:italic>\n                    NMT as a viable drug target. NMTs are secreted in all life stages of malarial parasites, making them attractive targets, unlike current antimalarials that are only effective during the plasmodial erythrocytic stages. The 2.3\u2005\u00c5 resolution crystal structure of the ternary complex of\n                    <jats:italic>Pv<\/jats:italic>\n                    NMT with myristoyl-CoA and a novel inhibitor is reported. One asymmetric unit contains two monomers. The structure reveals notable differences between the\n                    <jats:italic>Pv<\/jats:italic>\n                    NMT and human enzymes and similarities to other plasmodial NMTs that can be exploited to develop new antimalarials.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x24008604","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T07:13:53Z","timestamp":1726125233000},"page":"269-277","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Ternary structure of\n                    <i>Plasmodium vivax<\/i>\n                    <i>N<\/i>\n                    -myristoyltransferase with myristoyl-CoA and inhibitor IMP-0001173"],"prefix":"10.1107","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9996-5693","authenticated-orcid":true,"given":"Cydni","family":"Bolling","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05fde5z47","id-type":"ROR","asserted-by":"publisher"}],"department":["Chemistry and Biochemistry Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Mendez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05fde5z47","id-type":"ROR","asserted-by":"publisher"}],"department":["Chemistry and Biochemistry Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane","family":"Taylor","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05fde5z47","id-type":"ROR","asserted-by":"publisher"}],"department":["Chemistry and Biochemistry Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4355-1382","authenticated-orcid":true,"given":"Stanley","family":"Makumire","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03yj89h83","id-type":"ROR","asserted-by":"publisher"}],"department":["Structural Biology Research Unit, Faculty of Biochemistry and Molecular Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Reers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Zigweid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhya","family":"Subramanian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Dranow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Staker","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03yj89h83","id-type":"ROR","asserted-by":"publisher"}],"department":["Structural Biology Research Unit, Faculty of Biochemistry and Molecular Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward W.","family":"Tate","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S.","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Myler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4043-2700","authenticated-orcid":true,"given":"Oluwatoyin A.","family":"Asojo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/049s0rh22","id-type":"ROR","asserted-by":"publisher"}],"department":["Dartmouth Cancer Center"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4539-3971","authenticated-orcid":true,"given":"Graham","family":"Chakafana","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05fde5z47","id-type":"ROR","asserted-by":"publisher"}],"department":["Chemistry and Biochemistry Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2024,9,18]]},"reference":[{"key":"ir5034_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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