{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:14:22Z","timestamp":1785428062676,"version":"3.56.0"},"reference-count":72,"publisher":"International Union of Crystallography (IUCr)","issue":"3","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["75N93022C00036"],"award-info":[{"award-number":["75N93022C00036"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006132","name":"U.S. Department of Energy, Office of Science","doi-asserted-by":"publisher","award":["DE-AC02-06CH11357"],"award-info":[{"award-number":["DE-AC02-06CH11357"]}],"id":[{"id":"10.13039\/100006132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004948","name":"Michigan Economic Development Corporation","doi-asserted-by":"publisher","award":["085P1000817"],"award-info":[{"award-number":["085P1000817"]}],"id":[{"id":"10.13039\/100004948","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2025,1,29]]},"published-print":{"date-parts":[[2025,3,1]]},"abstract":"<jats:p>\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    is the primary causative agent of peptic ulcer disease, among other gastrointestinal ailments, and currently affects over half of the global population. Although some treatments exist, growing resistance to these drugs has prompted efforts to develop novel approaches to fighting this pathogen. To generate many of the nucleotides essential to biochemical processes,\n                    <jats:italic>H. pylori<\/jats:italic>\n                    relies exclusively on the\n                    <jats:italic>de novo<\/jats:italic>\n                    biosynthesis of these molecules. Recent drug-discovery efforts have targeted the first committed step of this pathway, catalysed by a class 2 dihydroorotate dehydrogenase (DHODH). However, these initiatives have been limited by the lack of a crystal structure. Here, we detail the crystal structure of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    DHODH (\n                    <jats:italic>Hp<\/jats:italic>\n                    DHODH) at 2.25\u2005\u00c5 resolution (PDB entry 6b8s). We performed a large-scale bioinformatics search to find evolutionary homologs. Our results indicate that\n                    <jats:italic>Hp<\/jats:italic>\n                    DHODH shows high conservation of both sequence and structure in its active site. We identified key polar interactions between the\n                    <jats:italic>Hp<\/jats:italic>\n                    DHODH protein and its requisite flavin mononucleotide (FMN) cofactor, identifying amino-acid residues that are critical to its function. Most notably, we found that\n                    <jats:italic>Hp<\/jats:italic>\n                    DHODH maintains several structural features that allow it to associate with the inner membrane and utilize ubiquinone to achieve catalytic turnover. We discovered a hydrophobic channel that runs from the putative membrane interface on the N-terminal microdomain to the core of the protein. We predict that this channel establishes a connection between the ubiquinone pool in the membrane and the FMN in the active site. These findings provide a structural explanation for the competitive inhibition of ubiquinone by pyrazole-based compounds that was determined biochemically in other studies. Understanding this mechanism may facilitate the development of new drugs targeting this enzyme and push the effort to find a resistance-free treatment for\n                    <jats:italic>H. pylori<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1107\/s2053230x25000858","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T10:56:47Z","timestamp":1739271407000},"page":"108-117","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Crystal structure of dihydroorotate dehydrogenase from\n                    <i>Helicobacter pylori<\/i>\n                    with bound flavin mononucleotide"],"prefix":"10.1107","volume":"81","author":[{"given":"Ashna A.","family":"Agarwal","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04cf69335","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4783-1553","authenticated-orcid":true,"given":"John D.","family":"Georgiades","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04cf69335","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Dranow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9638-8083","authenticated-orcid":true,"given":"Donald D.","family":"Lorimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayleigh F.","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin K.","family":"Craig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley C.","family":"Van Voorhis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-0513","authenticated-orcid":true,"given":"Peter J.","family":"Myler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3174-3053","authenticated-orcid":true,"given":"Craig L.","family":"Smith","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04cf69335","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"key":"cha5001_bb1","doi-asserted-by":"crossref","first-page":"7608","DOI":"10.1016\/j.tet.2013.05.033","volume":"69","author":"Abe","year":"2013","journal-title":"Tetrahedron"},{"key":"cha5001_bb101","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. D"},{"key":"cha5001_bb2","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1007\/s001099900067","volume":"77","author":"Alm","year":"1999","journal-title":"J. Mol. Med."},{"key":"cha5001_bb3","doi-asserted-by":"crossref","first-page":"17790","DOI":"10.1039\/C5CP02016F","volume":"17","author":"Alves","year":"2015","journal-title":"Phys. Chem. Chem. Phys."},{"key":"cha5001_bb4","doi-asserted-by":"crossref","first-page":"6069","DOI":"10.1093\/nar\/18.20.6069","volume":"18","author":"Aslanidis","year":"1990","journal-title":"Nucleic Acids Res."},{"key":"cha5001_bb5","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/0006-291X(76)90972-4","volume":"72","author":"Banner","year":"1976","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"cha5001_bb6","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/BF01232751","volume":"19","author":"Barnes","year":"1993","journal-title":"Somat. Cell Mol. Genet."},{"key":"cha5001_bb7","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","volume":"28","author":"Berman","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"cha5001_bb8","doi-asserted-by":"crossref","first-page":"2899","DOI":"10.1021\/bi982352c","volume":"38","author":"Bj\u00f6rnberg","year":"1999","journal-title":"Biochemistry"},{"key":"cha5001_bb9","doi-asserted-by":"crossref","first-page":"16197","DOI":"10.1021\/bi971628y","volume":"36","author":"Bj\u00f6rnberg","year":"1997","journal-title":"Biochemistry"},{"key":"cha5001_bb10","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1107\/S1744309111018367","volume":"67","author":"Bryan","year":"2011","journal-title":"Acta Cryst. F"},{"key":"cha5001_bb11","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/ijch.201200096","volume":"53","author":"Celniker","year":"2013","journal-title":"Isr. J. Chem."},{"key":"cha5001_bb99","doi-asserted-by":"crossref","first-page":"5449","DOI":"10.1093\/bioinformatics\/btac680","volume":"38","author":"Chatzigoulas","year":"2022","journal-title":"Bioinformatics"},{"key":"cha5001_bb98","doi-asserted-by":"crossref","first-page":"bbab518","DOI":"10.1093\/bib\/bbab518","volume":"23","author":"Chatzigoulas","year":"2022","journal-title":"Brief. Bioinform."},{"key":"cha5001_bb12","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/0003-9861(76)90143-0","volume":"176","author":"Chen","year":"1976","journal-title":"Arch. Biochem. Biophys."},{"key":"cha5001_bb13","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1107\/S0907444909042073","volume":"66","author":"Chen","year":"2010","journal-title":"Acta Cryst. D"},{"key":"cha5001_bb14","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1107\/S1744309111017374","volume":"67","author":"Choi","year":"2011","journal-title":"Acta Cryst. F"},{"key":"cha5001_bb15","doi-asserted-by":"crossref","first-page":"33373","DOI":"10.1074\/jbc.M004451200","volume":"275","author":"Copeland","year":"2000","journal-title":"J. Biol. Chem."},{"key":"cha5001_bb53","unstructured":"DeLano, W. L. (2002). PyMOL. http:\/\/www.pymol.org."},{"key":"cha5001_bb16","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1128\/MMBR.63.3.675-707.1999","volume":"63","author":"Doig","year":"1999","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"cha5001_bb17","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1128\/CMR.10.4.720","volume":"10","author":"Dunn","year":"1997","journal-title":"Clin. Microbiol. Rev."},{"key":"cha5001_bb18","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"cha5001_bb19","doi-asserted-by":"crossref","first-page":"14926","DOI":"10.1021\/bi060919g","volume":"45","author":"Fagan","year":"2006","journal-title":"Biochemistry"},{"key":"cha5001_bb20","doi-asserted-by":"crossref","first-page":"7169","DOI":"10.1021\/bi900370s","volume":"48","author":"Fagan","year":"2009","journal-title":"Biochemistry"},{"key":"cha5001_bb21","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1107\/S0021889809042022","volume":"42","author":"Gorrec","year":"2009","journal-title":"J. Appl. Cryst."},{"key":"cha5001_bb22","first-page":"20220408487609","author":"Hallgren","year":"2022","journal-title":"bioRxiv"},{"key":"cha5001_bb23","doi-asserted-by":"crossref","first-page":"4669","DOI":"10.1021\/jm020112w","volume":"45","author":"Haque","year":"2002","journal-title":"J. Med. Chem."},{"key":"cha5001_bb24","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.bbabio.2005.08.007","volume":"1710","author":"Hauss","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"cha5001_bb25","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1021\/bi00429a041","volume":"28","author":"Hines","year":"1989","journal-title":"Biochemistry"},{"key":"cha5001_bb26","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/pro.3749","volume":"29","author":"Holm","year":"2020","journal-title":"Protein Sci."},{"key":"cha5001_bb27","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1053\/j.gastro.2017.04.022","volume":"153","author":"Hooi","year":"2017","journal-title":"Gastroenterology"},{"key":"cha5001_bb28","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/pro.3280","volume":"27","author":"Jurrus","year":"2018","journal-title":"Protein Sci."},{"key":"cha5001_bb29","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"cha5001_bb30","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1006\/abbi.1999.1455","volume":"371","author":"Kahler","year":"1999","journal-title":"Arch. Biochem. Biophys."},{"key":"cha5001_bb31","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/ja.2013.42","volume":"66","author":"Kawada","year":"2013","journal-title":"J. Antibiot."},{"key":"cha5001_bb32","doi-asserted-by":"crossref","first-page":"9801","DOI":"10.1021\/bi901024m","volume":"48","author":"Kow","year":"2009","journal-title":"Biochemistry"},{"key":"cha5001_bb33","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1074\/jbc.M111.315267","volume":"287","author":"Lee","year":"2012","journal-title":"J. Biol. Chem."},{"key":"cha5001_bb34","doi-asserted-by":"crossref","first-page":"D435","DOI":"10.1093\/nar\/gkx1069","volume":"46","author":"Lewis","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"cha5001_bb35","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","volume":"75","author":"Liebschner","year":"2019","journal-title":"Acta Cryst. D"},{"key":"cha5001_bb36","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1128\/JB.05757-11","volume":"194","author":"Liechti","year":"2012","journal-title":"J. Bacteriol."},{"key":"cha5001_bb37","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0969-2126(00)00077-0","volume":"8","author":"Liu","year":"2000","journal-title":"Structure"},{"key":"cha5001_bb38","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S0006-2952(00)00348-8","volume":"60","author":"Marcinkeviciene","year":"2000","journal-title":"Biochem. Pharmacol."},{"key":"cha5001_bb39","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. Cryst."},{"key":"cha5001_bb40","doi-asserted-by":"crossref","first-page":"3148","DOI":"10.1021\/jm301848w","volume":"56","author":"Munier-Lehmann","year":"2013","journal-title":"J. Med. Chem."},{"key":"cha5001_bb41","doi-asserted-by":"crossref","first-page":"493","DOI":"10.2174\/187152609789105687","volume":"9","author":"Myler","year":"2009","journal-title":"Infect. Disord. Drug Targets"},{"key":"cha5001_bb42","doi-asserted-by":"crossref","first-page":"8966","DOI":"10.1073\/pnas.89.19.8966","volume":"89","author":"Nagy","year":"1992","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"cha5001_bb43","first-page":"183","volume":"1","author":"Neidhardt","year":"1999","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"cha5001_bb44","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1107\/S0907444905024984","volume":"61","author":"Newman","year":"2005","journal-title":"Acta Cryst. D"},{"key":"cha5001_bb45","doi-asserted-by":"crossref","first-page":"29359","DOI":"10.1074\/jbc.271.46.29359","volume":"271","author":"Nielsen","year":"1996","journal-title":"J. Biol. Chem."},{"key":"cha5001_bb46","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.biochi.2019.01.006","volume":"158","author":"Nonato","year":"2019","journal-title":"Biochimie"},{"key":"cha5001_bb47","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/S0969-2126(02)00831-6","volume":"10","author":"N\u00f8rager","year":"2002","journal-title":"Structure"},{"key":"cha5001_bb48","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.copbio.2017.03.010","volume":"48","author":"Okesli","year":"2017","journal-title":"Curr. Opin. Biotechnol."},{"key":"cha5001_bb51","doi-asserted-by":"crossref","unstructured":"Orozco Rodriguez, J. M., Wacklin-Knecht, H. P., Clifton, L. A., Bogojevic, O., Leung, A., Fragneto, G. & Knecht, W. (2022). Int. J. Mol. Sci. 23, 2437.","DOI":"10.3390\/ijms23052437"},{"key":"cha5001_bb49","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0304-4173(85)90003-5","volume":"811","author":"Ragan","year":"1985","journal-title":"Biochim. Biophys. Acta"},{"key":"cha5001_bb50","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1046\/j.1432-1327.2000.01213.x","volume":"267","author":"Rawls","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"cha5001_bb52","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/BF00231812","volume":"128","author":"Samor\u00ec","year":"1992","journal-title":"J. Membr. Biol."},{"key":"cha5001_bb54","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1107\/S2053230X15004677","volume":"71","author":"Serbzhinskiy","year":"2015","journal-title":"Acta Cryst. F"},{"key":"cha5001_bb55","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.mib.2009.11.006","volume":"13","author":"Shiloh","year":"2010","journal-title":"Curr. Opin. Microbiol."},{"key":"cha5001_bb57","doi-asserted-by":"crossref","first-page":"D266","DOI":"10.1093\/nar\/gkaa1079","volume":"49","author":"Sillitoe","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"cha5001_bb58","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jcis.2004.02.012","volume":"275","author":"Sondi","year":"2004","journal-title":"J. Colloid Interface Sci."},{"key":"cha5001_bb59","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s00792-001-0249-0","volume":"6","author":"S\u00f8rensen","year":"2002","journal-title":"Extremophiles"},{"key":"cha5001_bb60","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1107\/S1744309111029204","volume":"67","author":"Stacy","year":"2011","journal-title":"Acta Cryst. F"},{"key":"cha5001_bb61","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pep.2005.01.016","volume":"41","author":"Studier","year":"2005","journal-title":"Protein Expr. Purif."},{"key":"cha5001_bb62","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1056\/NEJMra020542","volume":"347","author":"Suerbaum","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"cha5001_bb63","doi-asserted-by":"crossref","first-page":"12619","DOI":"10.1073\/pnas.95.21.12619","volume":"95","author":"Suerbaum","year":"1998","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"cha5001_bb64","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.3748\/wjg.v25.i16.1907","volume":"25","author":"Suzuki","year":"2019","journal-title":"World J. Gastroenterol."},{"key":"cha5001_bb65","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","volume":"18","author":"Tacconelli","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"cha5001_bb66","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1128\/jb.88.1.105-110.1964","volume":"88","author":"Taylor","year":"1964","journal-title":"J. Bacteriol."},{"key":"cha5001_bb67","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1038\/s41575-021-00449-x","volume":"18","author":"Tshibangu-Kabamba","year":"2021","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"cha5001_bb68","doi-asserted-by":"crossref","first-page":"8929","DOI":"10.1021\/bi8003318","volume":"47","author":"Walse","year":"2008","journal-title":"Biochemistry"},{"key":"cha5001_bb69","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Cryst. D"},{"key":"cha5001_bb70","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0304-4173(81)90007-0","volume":"639","author":"Yu","year":"1981","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2025\/03\/00\/cha5001\/cha5001.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2025\/03\/00\/cha5001\/cha5001.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:32:26Z","timestamp":1761309146000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X25000858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,17]]},"references-count":72,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["S2053230X25000858"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x25000858","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1107\/S2053230X25000858\/cha5001sup1.pdf","asserted-by":"subject"},{"id-type":"doi","id":"10.1107\/S2053230X25000858\/cha5001sup2.mp4","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb6b8s\/pdb","asserted-by":"subject"}]},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,17]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?cha5001","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"1 December 2024","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"29 January 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"17 February 2025","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?cha5001"}},{"value":"\u00a9 Ashna A. Agarwal\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}},{"value":"IUCr licence to publish","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/journals.iucr.org\/copyright\/licencetopublish.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}