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This disease is a growing health threat due to the occurrence of multidrug resistance. Mycolic acids are essential for generating cell walls and their modification is important to the virulence and persistence of\n                    <jats:italic>M. tuberculosis.<\/jats:italic>\n                    A family of\n                    <jats:italic>S<\/jats:italic>\n                    -adenosylmethionine-dependent mycolic acid synthases modify mycolic acids and represent promising drug targets. UmaA is currently the least-understood member of this family. This paper describes the crystal structure of UmaA. UmaA is a monomer composed of two domains: a structurally conserved SAM-binding domain and a variable substrate-binding auxiliary domain. Fortuitously, our structure contains a nitrate in the active site, a structural mimic of carbonate, which is a known general base in cyclopropane-adding synthases. Further investigation indicated that the structure of the N-terminus is highly flexible. Finally, we have identified\n                    <jats:italic>S<\/jats:italic>\n                    -adenosyl-\n                    <jats:italic>N<\/jats:italic>\n                    -decyl-aminoethyl as a promising potential inhibitor.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x25001530","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T10:42:24Z","timestamp":1741257744000},"page":"146-154","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Crystal structure of the\n                    <i>S<\/i>\n                    -adenosylmethionine-dependent mycolic acid synthase UmaA from\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1107","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0889-616X","authenticated-orcid":true,"given":"Sean","family":"Teng","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yc7t268","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2683-8761","authenticated-orcid":true,"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yc7t268","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Collin D.","family":"Sroge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-3243","authenticated-orcid":true,"given":"Jan","family":"Abendroth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9638-8083","authenticated-orcid":true,"given":"Donald D.","family":"Lorimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S.","family":"Horanyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Logan","family":"Tillery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin K.","family":"Craig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley C.","family":"Van Voorhis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-0513","authenticated-orcid":true,"given":"Peter J.","family":"Myler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3174-3053","authenticated-orcid":true,"given":"Craig L.","family":"Smith","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01yc7t268","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"ir5043_bb2","doi-asserted-by":"crossref","first-page":"6069","DOI":"10.1093\/nar\/18.20.6069","volume":"18","author":"Aslanidis","year":"1990","journal-title":"Nucleic Acids Res."},{"key":"ir5043_bb3","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.chembiol.2009.04.001","volume":"16","author":"Barkan","year":"2009","journal-title":"Chem. 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