{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:02:50Z","timestamp":1785402170035,"version":"3.56.0"},"reference-count":48,"publisher":"International Union of Crystallography (IUCr)","issue":"4","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/100001309","name":"Research Corporation for Science Advancement","doi-asserted-by":"publisher","award":["28277"],"award-info":[{"award-number":["28277"]}],"id":[{"id":"10.13039\/100001309","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2025,2,25]]},"published-print":{"date-parts":[[2025,4,1]]},"abstract":"<jats:p>\n                    Cellular deoxyuridine 5\u2032-triphosphate nucleotidohydrolases (dUTPases) catalyze the hydrolysis of deoxyuridine triphosphate (dUTP) to deoxyuridine monophosphate (dUMP) and pyrophosphate (PP\n                    <jats:sub>i<\/jats:sub>\n                    ). dUTPase is an essential metabolic enzyme which maintains the homeostatic dTTP:dUTP ratio. As DNA polymerases are unable to distinguish between thymine and uracil during replication, the dTTP:dUTP ratio is essential for preventing the misincorporation of uracil into DNA. In the absence of dUTPase regulation of the dTTP:dUTP ratio, many DNA double-strand breaks are induced by DNA-repair enzymes, which may ultimately lead to cell death. Legionnaires' disease is a rare but severe respiratory infection caused primarily by\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    serogroup 1. Increased characterization of the\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    proteome is of interest for the development of new treatments. Many DNA metabolism proteins have yet to be characterized in\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    , including dUTPase. Here, we present analysis of two crystal structures of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    dUTPase in its apo and dUMP-bound states, determined to 1.80 and 1.95\u2005\u00c5 resolution, respectively. The structures were solved by the Seattle Structural Genomics Center for Infectious Disease (SSGCID) as part of their mission to determine structures of proteins and other molecules with an important biological role in human pathogens.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x25001815","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T10:50:57Z","timestamp":1741690257000},"page":"155-162","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Structural characterization of dUTPase from\n                    <i>Legionella pneumophila<\/i>"],"prefix":"10.1107","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0914-3801","authenticated-orcid":true,"given":"Chi L.","family":"Nguyen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail R.","family":"Tramell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan O.","family":"Norman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-3243","authenticated-orcid":true,"given":"Jan","family":"Abendroth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayleigh F.","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin K.","family":"Craig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0474-8003","authenticated-orcid":true,"given":"Thomas E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9638-8083","authenticated-orcid":true,"given":"Donald D.","family":"Lorimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6141-2015","authenticated-orcid":true,"given":"Wesley C.","family":"Van Voorhis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ymk3103","id-type":"ROR","asserted-by":"publisher"}],"department":["Center for Emerging and Re-emerging Infectious Diseases (CERID), Department of Medicine, Division of Allergy and Infectious Diseases"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4105-1042","authenticated-orcid":true,"given":"Krystle J.","family":"McLaughlin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"ir5042_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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Legionellosis. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/legionellosis."}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2025\/04\/00\/ir5042\/ir5042.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2025\/04\/00\/ir5042\/ir5042.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T14:34:54Z","timestamp":1752330894000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X25001815"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,17]]},"references-count":48,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["S2053230X25001815"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x25001815","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1107\/S2053230X25001815\/ir5042sup1.pdf","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb6mai\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb6mao\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.18430\/m36mai","asserted-by":"subject"},{"id-type":"doi","id":"10.18430\/m36mao","asserted-by":"subject"}]},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,17]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?ir5042","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"15 November 2024","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"25 February 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"17 March 2025","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?ir5042"}},{"value":"\u00a9 Chi L. Nguyen\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}},{"value":"IUCr licence to publish","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/journals.iucr.org\/copyright\/licencetopublish.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}