{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:17:56Z","timestamp":1785403076626,"version":"3.56.0"},"reference-count":37,"publisher":"International Union of Crystallography (IUCr)","issue":"4","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/100001309","name":"Research Corporation for Science Advancement","doi-asserted-by":"publisher","award":["28277"],"award-info":[{"award-number":["28277"]}],"id":[{"id":"10.13039\/100001309","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2025,2,27]]},"published-print":{"date-parts":[[2025,4,1]]},"abstract":"<jats:p>\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    serogroup 1 is the primary causative agent of Legionnaires' disease, a rare but severe respiratory infection. While the fatality rate of Legionnaires' disease is low in the general population, it is more pronounced in vulnerable communities such as the immunocompromised. Thus, the development of new antimicrobials is of interest for use when existing antibiotics may not be applicable. Peptide deformylases (PDFs) have been under continued investigation as targets for novel antimicrobial compounds. PDF plays an essential role in protein synthesis, removing the N-terminal formyl group from new polypeptides, and is required for growth in most bacteria. Here, we report two crystal structures of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    serogroup 1 PDF (LpPDF) bound to either Ni\n                    <jats:sup>2+<\/jats:sup>\n                    , an active state, or inhibited by actinonin and Zn\n                    <jats:sup>2+<\/jats:sup>\n                    ; the structures were determined to 1.5 and 1.65\u2005\u00c5 resolution, respectively, and were solved by the Seattle Structural Genomics Center for Infectious Disease (SSGCID). The SSGCID is charged with determining structures of biologically important proteins and molecules from human pathogens. As actinonin is an antimicrobial natural product that has been used as a reference compound in drug development, these structures will help support the ongoing drug-development process.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x25001876","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T11:07:41Z","timestamp":1741777661000},"page":"163-170","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Structures of\n                    <i>Legionella pneumophila<\/i>\n                    serogroup 1 peptide deformylase bound to nickel(II) and actinonin"],"prefix":"10.1107","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0914-3801","authenticated-orcid":true,"given":"Chi L.","family":"Nguyen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Fan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Fisher","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystal","family":"Matthews","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Chemistry Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan O.","family":"Norman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-3243","authenticated-orcid":true,"given":"Jan","family":"Abendroth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayleigh F.","family":"Barrett","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ymk3103","id-type":"ROR","asserted-by":"publisher"}],"department":["Center for Emerging and Re-emerging Infectious Diseases (CERID), Department of Medicine, Division of Allergy and Infectious Diseases"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin K.","family":"Craig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0474-8003","authenticated-orcid":true,"given":"Thomas E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9638-8083","authenticated-orcid":true,"given":"Donald D.","family":"Lorimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4105-1042","authenticated-orcid":true,"given":"Krystle J.","family":"McLaughlin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022x6qg61","id-type":"ROR","asserted-by":"publisher"}],"department":["Biochemistry Program"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"ir5044_bb1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"ir5044_bb2","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1128\/AAC.45.4.1058-1064.2001","volume":"45","author":"Apfel","year":"2001","journal-title":"Antimicrob. 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Nguyen\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licensing","label":"Copyright and licencing"}},{"value":"IUCr licence to publish","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/journals.iucr.org\/copyright\/licencetopublish.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}