{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:55:42Z","timestamp":1785783342333,"version":"3.56.0"},"reference-count":19,"publisher":"International Union of Crystallography (IUCr)","issue":"8","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2025,6,11]]},"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:p>Nucleotide-bound crystal structures of SARS-CoV-2 NSP13 in ADP- and ATP-bound states were resolved to 1.8 and 1.9\u2005\u00c5, respectively. The ADP-bound model captures a state immediately following ATP hydrolysis, with both ADP and orthophosphate still present in the active site. Further comparative analysis revealed that crystal packing influences NSP13 by stabilizing the nucleotide-binding site, underscoring the importance of accounting for these effects in structure-based drug design targeting NSP13.<\/jats:p>","DOI":"10.1107\/s2053230x25005266","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T04:34:42Z","timestamp":1750221282000},"page":"338-347","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":4,"title":["Nucleotide-bound crystal structures of the SARS-CoV-2 helicase NSP13"],"prefix":"10.1107","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7077-0747","authenticated-orcid":true,"given":"Patrick","family":"Kloskowski","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology, Institute of Microbiology and Genetics, G\u00f6ttingen Center of Molecular Biosciences (GZMB)"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Neumann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology, Institute of Microbiology and Genetics, G\u00f6ttingen Center of Molecular Biosciences (GZMB)"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Berndt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology, Institute of Microbiology and Genetics, G\u00f6ttingen Center of Molecular Biosciences (GZMB)"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1739-6086","authenticated-orcid":true,"given":"Ralf","family":"Ficner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y9bpm73","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Structural Biology, Institute of Microbiology and Genetics, G\u00f6ttingen Center of Molecular Biosciences (GZMB)"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,7,10]]},"reference":[{"key":"no5212_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. D"},{"key":"no5212_bb2","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1016\/j.cell.2020.07.033","volume":"182","author":"Chen","year":"2020","journal-title":"Cell"},{"key":"no5212_bb3","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/s41594-022-00734-6","volume":"29","author":"Chen","year":"2022","journal-title":"Nat. Struct. Mol. Biol."},{"key":"no5212_bb4","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"no5212_bb5","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1107\/S0907444905036693","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Cryst. D"},{"key":"no5212_bb6","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1107\/S0907444913000061","volume":"69","author":"Evans","year":"2013","journal-title":"Acta Cryst. D"},{"key":"no5212_bb7","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.sbi.2010.03.011","volume":"20","author":"Fairman-Williams","year":"2010","journal-title":"Curr. Opin. Struct. Biol."},{"key":"no5212_bb8","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1107\/S2053230X24007039","volume":"80","author":"Garbers","year":"2024","journal-title":"Acta Cryst. F"},{"key":"no5212_bb9","doi-asserted-by":"crossref","first-page":"199401","DOI":"10.1016\/j.virusres.2024.199401","volume":"346","author":"Grimes","year":"2024","journal-title":"Virus Res."},{"key":"no5212_bb10","doi-asserted-by":"crossref","first-page":"e4519","DOI":"10.1002\/pro.4519","volume":"32","author":"Holm","year":"2023","journal-title":"Protein Sci."},{"key":"no5212_bb11","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.jmb.2016.12.004","volume":"429","author":"Jubb","year":"2017","journal-title":"J. Mol. Biol."},{"key":"no5212_bb12","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Cryst. D"},{"key":"no5212_bb13","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1107\/S2059798325004498","volume":"81","author":"Kloskowski","year":"2025","journal-title":"Acta Cryst. D"},{"key":"no5212_bb14","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1107\/S0907444911001314","volume":"67","author":"Murshudov","year":"2011","journal-title":"Acta Cryst. D"},{"key":"no5212_bb15","doi-asserted-by":"crossref","first-page":"4848","DOI":"10.1038\/s41467-021-25166-6","volume":"12","author":"Newman","year":"2021","journal-title":"Nat. Commun."},{"key":"no5212_bb16","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/978-1-4614-5037-5_2","volume":"767","author":"Raney","year":"2013","journal-title":"Adv. Exp. Med. Biol."},{"key":"no5212_bb17","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1107\/S0907444911007773","volume":"67","author":"Vonrhein","year":"2011","journal-title":"Acta Cryst. D"},{"key":"no5212_bb18","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Cryst. D"},{"key":"no5212_bb19","doi-asserted-by":"crossref","first-page":"s299","DOI":"10.1107\/S0108767313097419","volume":"69","author":"Wojdyr","year":"2013","journal-title":"Acta Cryst. A"}],"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2025\/08\/00\/no5212\/no5212.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2025\/08\/00\/no5212\/no5212.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T12:10:22Z","timestamp":1753963822000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X25005266"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,10]]},"references-count":19,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["S2053230X25005266"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x25005266","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.2210\/pdb9i51\/pdb","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9i53\/pdb","asserted-by":"subject"}]},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,10]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?no5212","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"14 February 2025","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"11 June 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"10 July 2025","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?no5212"}},{"value":"\u00a9 2025 Patrick Kloskowski\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}