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Here, we report the apo and NAD\n                    <jats:sup>+<\/jats:sup>\n                    -bound structures of an SDR from the pathogenic organism\n                    <jats:italic>Brucella ovis<\/jats:italic>\n                    .\n                    <jats:italic>B. ovis<\/jats:italic>\n                    primarily affects sheep and other livestock, resulting in reduced fertility. Based on sequence and structural alignment, the\n                    <jats:italic>B.\u00a0ovis<\/jats:italic>\n                    SDR (BoSDR) is a classical SDR. Classical SDRs have a canonical Y\n                    <jats:italic>xxx<\/jats:italic>\n                    K active-site sequence in which the catalytic general base is a tyrosine residue located at position 163. In addition, the putative active site also contains a serine residue (Ser150) and lysine residue (Lys167) that are hypothesized to be involved in catalysis. BoSDR is a biological and crystallographic tetramer. In the coenzyme-bound structure, two different orientations of the NAD\n                    <jats:sup>+<\/jats:sup>\n                    coenzyme are fortuitously observed, which provides insights into the conformational changes that accompany coenzyme binding. The apo and NAD\n                    <jats:sup>+<\/jats:sup>\n                    -bound structures provide valuable information about the unique structural features of enzymes in the SDR superfamily.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x25009227","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T10:58:31Z","timestamp":1762858711000},"page":"487-494","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystal structure of a short-chain dehydrogenase from\n                    <i>Brucella ovis<\/i>\n                    with apo and coenzyme NAD\n                    <sup>+<\/sup>\n                    -bound protomer chains"],"prefix":"10.1107","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8568-5434","authenticated-orcid":true,"given":"Sean P.","family":"Zupko","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a4pj207","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3130-1231","authenticated-orcid":true,"given":"Amelia T.","family":"Konstanty","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a4pj207","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1600-4554","authenticated-orcid":true,"given":"Steve J.","family":"Mayclin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9374-5893","authenticated-orcid":true,"given":"Ryan","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Serbzhinskiy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9410-7236","authenticated-orcid":true,"given":"Emily","family":"Robles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Moses","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/014wfj781","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn K.","family":"Barrett","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00cvxb145","id-type":"ROR","asserted-by":"publisher"}],"department":["Division of Allergy and Infectious Diseases, Department of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6141-2015","authenticated-orcid":true,"given":"Wesley C.","family":"Van Voorhis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0474-8003","authenticated-orcid":true,"given":"Tom E.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-0513","authenticated-orcid":true,"given":"Peter J.","family":"Myler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4130-9867","authenticated-orcid":true,"given":"Andrew T.","family":"Torelli","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01kw1gj07","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6762-1309","authenticated-orcid":true,"given":"Jarrod B.","family":"French","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/017zqws13","id-type":"ROR","asserted-by":"publisher"}],"department":["The Hormel Institute"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A.","family":"Hicks","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a4pj207","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2025,11,11]]},"reference":[{"key":"rf5049_bb1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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