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The development of a synthetic Fab against the BRIL domain improved the accessibility of this approach, providing a general fiducial applicable to any protein of interest via the simple curation of a BRIL fusion protein. Here, we document the generation of a T7 Express \u0394\n                    <jats:italic>cybC<\/jats:italic>\n                    strain allowing contaminant-free bacterial expression of the synthetic anti-BRIL Fab BAG2. We also report the crystal structure of BAG2 in complex with native cytochrome\n                    <jats:italic>b<\/jats:italic>\n                    <jats:sub>562<\/jats:sub>\n                    , a complex arising from expression in canonical\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    strains.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26001548","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T13:55:09Z","timestamp":1773755709000},"page":"143-149","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Optimized bacterial expression of a synthetic BRIL antibody"],"prefix":"10.1107","volume":"82","author":[{"given":"Benjamin F.","family":"Cooper","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"department":["Sir William Dunn School of Pathology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia L.","family":"Isom","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"department":["Sir William Dunn School of Pathology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"bus5006_bb2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev-biochem-032620-110705","volume":"91","author":"Chua","year":"2022","journal-title":"Annu. 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M., Radziwon, K., Coukos, J., Kang, Y., Dutka, P., Gao, X., Qiu, X., Yeager, M., Xu, H. E., Han, S. & Kossiakoff, A. A. (2020). Nat. Commun. 11, 1598.","DOI":"10.1038\/s41467-020-15363-0"},{"key":"bus5006_bb17","doi-asserted-by":"crossref","first-page":"2707","DOI":"10.1016\/j.jmb.2015.06.018","volume":"427","author":"Mukherjee","year":"2015","journal-title":"J. Mol. Biol."},{"key":"bus5006_bb18","doi-asserted-by":"crossref","first-page":"5686","DOI":"10.1038\/s41467-020-19458-6","volume":"11","author":"Pan","year":"2020","journal-title":"Nat. Commun."},{"key":"bus5006_bb19","doi-asserted-by":"crossref","first-page":"9241","DOI":"10.1038\/s41467-024-53533-6","volume":"15","author":"Shionoya","year":"2024","journal-title":"Nat. Commun."},{"key":"bus5006_bb20","doi-asserted-by":"crossref","first-page":"9936","DOI":"10.1038\/s41467-024-54219-9","volume":"15","author":"Sverak","year":"2024","journal-title":"Nat. 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