{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:27:54Z","timestamp":1785421674117,"version":"3.56.0"},"reference-count":0,"publisher":"International Union of Crystallography (IUCr)","issue":"5","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100020326","name":"Universidad Nacional de San Agustin de Arequipa","doi-asserted-by":"publisher","award":["IBA-22"],"award-info":[{"award-number":["IBA-22"]}],"id":[{"id":"10.13039\/501100020326","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["310754\/2021-6"],"award-info":[{"award-number":["310754\/2021-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2026,3,12]]},"published-print":{"date-parts":[[2026,5,1]]},"abstract":"<jats:p>\n                    Snake-venom phospholipases A\n                    <jats:sub>2<\/jats:sub>\n                    (PLA\n                    <jats:sub>2<\/jats:sub>\n                    s) are small, structurally conserved enzymes that contribute significantly to the pathophysiology of envenomation. Here, we report the purification and crystal structure of an Asp49-PLA\n                    <jats:sub>2<\/jats:sub>\n                    isolated from the venom of\n                    <jats:italic>Lachesis muta<\/jats:italic>\n                    , a pit viper from the Peruvian Amazon. The enzyme was purified using ion-exchange and size-exclusion chromatography and exhibited phospholipase activity in a dose- and time-dependent egg-yolk degradation assay. Pure protein crystals were obtained in space group\n                    <jats:italic>P<\/jats:italic>\n                    6\n                    <jats:sub>2<\/jats:sub>\n                    22 and diffracted to 2.36\u2005\u00c5 resolution, with two molecules in the asymmetric unit. The structure reveals the canonical fold of catalytically active group II PLA\n                    <jats:sub>2<\/jats:sub>\n                    s, with a bound Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ion and a MES molecule in the active site of one monomer. Seven disulfide bonds stabilize the structure, although one bridge typically associated with the \u03b2-hairpin is absent and is replaced by a salt bridge as in other viperid PLA\n                    <jats:sub>2<\/jats:sub>\n                    s.\n                    <jats:italic>PISA<\/jats:italic>\n                    analysis suggests a potential tetrameric assembly composed of two\n                    <jats:italic>AB<\/jats:italic>\n                    dimers generating an interface between two\n                    <jats:italic>A<\/jats:italic>\n                    subunits (\n                    <jats:italic>A<\/jats:italic>\n                    \u2013\n                    <jats:italic>A<\/jats:italic>\n                    \u2032). Electrostatic surface mapping reveals a notable positively charged channel at the\n                    <jats:italic>A<\/jats:italic>\n                    \u2013\n                    <jats:italic>A<\/jats:italic>\n                    \u2032 interface, like that seen for a basic PLA\n                    <jats:sub>2<\/jats:sub>\n                    homodimer from\n                    <jats:italic>Crotalus durissus terrificus<\/jats:italic>\n                    in which the two active sites lie accessible to the membrane. This study presents the first structural and enzymatic analysis of an Asp49-PLA\n                    <jats:sub>2<\/jats:sub>\n                    from\n                    <jats:italic>L.\u00a0muta<\/jats:italic>\n                    and provides insights into its oligomeric assembly, electrostatic landscape and potential adaptations relevant to its role in venom toxicity.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26002736","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T14:26:53Z","timestamp":1774967213000},"page":"150-159","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystal structure and functional characterization of an Asp49 phospholipase A\n                    <sub>2<\/sub>\n                    from the bushmaster (\n                    <i>Lachesis muta<\/i>\n                    )"],"prefix":"10.1107","volume":"82","author":[{"given":"Noelia Erika","family":"Neyra Chama","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e9gfg41","id-type":"ROR","asserted-by":"publisher"}],"department":["Laboratorio de F\u00edsica Medica, Escuela Profesional de F\u00edsica"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4341-7941","authenticated-orcid":true,"given":"Frey Francisco","family":"Romero Vargas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e9gfg41","id-type":"ROR","asserted-by":"publisher"}],"department":["Laboratorio de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Ciencias Biol\u00f3gicas"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloy","family":"Condori Mamani","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de F\u00edsica de S\u00e3o Carlos"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhon Antoni","family":"Vargas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de F\u00edsica de S\u00e3o Carlos"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7565-3413","authenticated-orcid":true,"given":"Adriano","family":"Alves Furtado","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de F\u00edsica de S\u00e3o Carlos"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humberto","family":"D'Muniz Pereira","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de F\u00edsica de S\u00e3o Carlos"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7217-2926","authenticated-orcid":true,"given":"Ronald Demetrio","family":"Navarro Oviedo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e9gfg41","id-type":"ROR","asserted-by":"publisher"}],"department":["Laboratorio de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Ciencias Biol\u00f3gicas"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard Charles","family":"Garratt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de F\u00edsica de S\u00e3o Carlos"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5562-8378","authenticated-orcid":true,"given":"Jos\u00e9 Luis Javier","family":"Vega Ram\u00edrez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e9gfg41","id-type":"ROR","asserted-by":"publisher"}],"department":["Laboratorio de F\u00edsica Medica, Escuela Profesional de F\u00edsica"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4264-3074","authenticated-orcid":true,"given":"Diego Antonio","family":"Leonardo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"department":["Instituto de F\u00edsica de S\u00e3o Carlos"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,4,7]]},"container-title":["Acta Crystallographica Section F Structural Biology Communications"],"original-title":[],"link":[{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2026\/05\/00\/nq5002\/nq5002.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.iucr.org\/f\/issues\/2026\/05\/00\/nq5002\/nq5002.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:20:37Z","timestamp":1777468837000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2053230X26002736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,7]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["S2053230X26002736"],"URL":"https:\/\/doi.org\/10.1107\/s2053230x26002736","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1107\/S2053230X26002736\/nq5002sup1.pdf","asserted-by":"subject"},{"id-type":"doi","id":"10.2210\/pdb9mle\/pdb","asserted-by":"subject"}]},"ISSN":["2053-230X"],"issn-type":[{"value":"2053-230X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,7]]},"assertion":[{"value":"Acta Crystallographica Section F: Structural Biology Communications","order":0,"name":"journal","label":"Publication","explanation":{"URL":"https:\/\/journals.iucr.org\/f"},"group":{"name":"general","label":"General"}},{"value":"research communications","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/f\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"https:\/\/scripts.iucr.org\/cgi-bin\/citedin?nq5002","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"1 November 2025","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"12 March 2026","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"7 April 2026","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"https:\/\/scripts.iucr.org\/cgi-bin\/sendsup?nq5002"}},{"value":"\u00a9 2026 Noelia Erika Neyra Chama\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}