{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:58:41Z","timestamp":1785437921521,"version":"3.56.0"},"reference-count":27,"publisher":"International Union of Crystallography (IUCr)","issue":"6","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"name":"Japanese Vitamin B Research Committee"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2026,4,2]]},"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>\n                    \u03b3-Aminobutyric acid aminotransferase (GABA-T) is a pyridoxal 5\u2032-phosphate (PLP)-dependent enzyme that catalyzes \u03b3-aminobutyric acid (GABA) degradation in the mitochondrial GABA shunt. Plant GABA-Ts correspond to EC 2.6.1.96 and differ from mammalian and bacterial GABA-Ts (EC 2.6.1.19) in substrate specificity; however, their structural basis has remained unclear. Here, we report the crystal structure of GABA aminotransferase from\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    (\n                    <jats:italic>At<\/jats:italic>\n                    GABA-T) at 2.0\u2005\u00c5 resolution. Structural comparison using\n                    <jats:italic>Foldseek<\/jats:italic>\n                    indicates that\n                    <jats:italic>At<\/jats:italic>\n                    GABA-T belongs to the class III aminotransferase family. Comparison with a class III \u03c9-aminotransferase suggests that Arg423 located in the C-terminal region of\n                    <jats:italic>At<\/jats:italic>\n                    GABA-T functions as the primary anchor for the carboxyl group of GABA, together with residues forming a tunnel-shaped substrate-access pathway. In contrast to nonplant GABA aminotransferases, which utilize a conserved N-terminal arginine for substrate recognition,\n                    <jats:italic>At<\/jats:italic>\n                    GABA-T employs a distinct C-terminal arginine. These findings provide direct structural evidence for the classification of\n                    <jats:italic>At<\/jats:italic>\n                    GABA-T as a class III aminotransferase and reveal a distinct mode of substrate recognition in\n                    <jats:italic>At<\/jats:italic>\n                    GABA-T.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26003456","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:34:27Z","timestamp":1777372467000},"page":"208-215","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystal structure of the plant GABA aminotransferase\n                    <i>At<\/i>\n                    GABA-T from\n                    <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1107","volume":"82","author":[{"given":"Naofumi","family":"Okoda","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suguru","family":"Okuda","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1168-6656","authenticated-orcid":true,"given":"Kenta","family":"Tsutsumi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Itoh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Okamoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kawakami","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Sano","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/021r6aq66","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Chemistry and Biological Science, Faculty of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Kobata","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/021r6aq66","id-type":"ROR","asserted-by":"publisher"}],"department":["Graduate School of Pharmaceutical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4704-0603","authenticated-orcid":true,"given":"Koji","family":"Nagata","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,5,1]]},"reference":[{"key":"no5216_bb1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"no5216_bb2","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1107\/S0907444912001308","volume":"68","author":"Afonine","year":"2012","journal-title":"Acta Cryst. 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