{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:45:09Z","timestamp":1785422709952,"version":"3.56.0"},"reference-count":27,"publisher":"International Union of Crystallography (IUCr)","issue":"6","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["7700002944"],"award-info":[{"award-number":["7700002944"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18GS0316"],"award-info":[{"award-number":["18GS0316"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018787","name":"Institute for Protein Research, Osaka University","doi-asserted-by":"publisher","award":["CR-25-02"],"award-info":[{"award-number":["CR-25-02"]}],"id":[{"id":"10.13039\/501100018787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2026,4,8]]},"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>\n                    <jats:italic>Aeropyrum pernix<\/jats:italic>\n                    is a hyperthermophilic archaeon that possesses three proliferating cell nuclear antigen (PCNA) isoforms (ApePCNA1, ApePCNA2 and ApePCNA3) that form a heterotrimeric sliding clamp. To gain more detailed structural insights into this heterotrimeric assembly, we determined the crystal structures of ApePCNA1 and ApePCNA2. ApePCNA1 was crystallized under a new condition, and the 1.60\u2005\u00c5 resolution structure revealed a unique nonproline\n                    <jats:italic>cis<\/jats:italic>\n                    -peptide bond between Arg187 and Arg188, which was not deeply discussed in a previous report. The structure of ApePCNA2 was determined at 2.17\u2005\u00c5 resolution, and it forms a typical homotrimeric ring. In the cubic crystal form, its crystal packing shows an intriguing tetrahedral assembly of four trimers. Modeling the ApePCNA1\u2013ApePCNA2\u2013ApePCNA3 heterotrimer based on these structures suggests that the\n                    <jats:italic>cis<\/jats:italic>\n                    -peptide in ApePCNA1 induces significant steric hindrance at the subunit interface, leading to a symmetry-broken or distorted ring conformation rather than the canonical pseudo-threefold-symmetric assembly.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26003687","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T10:05:58Z","timestamp":1778061958000},"page":"216-221","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystal structures of PCNA1 and PCNA2 from\n                    <i>Aeropyrum pernix<\/i>\n                    : implications for a distorted heterotrimeric sliding clamp"],"prefix":"10.1107","volume":"82","author":[{"given":"Tong","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/059x21724","id-type":"ROR","asserted-by":"publisher"}],"department":["Integrated Graduate School of Medicine, Engineering and Agricultural Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonoko","family":"Ishino","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00p4k0j84","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9419-0826","authenticated-orcid":true,"given":"Yoshizumi","family":"Ishino","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00p4k0j84","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3411-7608","authenticated-orcid":true,"given":"Takuji","family":"Oyama","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/059x21724","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Biotechnology, Faculty of Life and Environmental Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"ek5042_bb1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"ek5042_bb2","first-page":"a010124","volume":"5","author":"Bell","year":"2013","journal-title":"Cold Spring Harb. 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