{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T19:25:59Z","timestamp":1793647559293,"version":"4.5.1"},"reference-count":39,"publisher":"International Union of Crystallography (IUCr)","issue":"8","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2023.18077.ICDT"],"award-info":[{"award-number":["2023.18077.ICDT"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2026,7,2]]},"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:p>\n                    The importance of human aldehyde oxidase (hAOX1) has increased in recent decades due to its involvement in drug metabolism. Inhibition studies involving hAOX1 are extensive and a common reducing agent, dithiothreitol (DTT), was recently found to inactivate the enzyme. However, in previous crystallographic studies of hAOX1, DTT was found to be essential for crystallization. To surpass this concern, another reducing agent was used in crystallization trials. Using tris(2-carboxyethyl)phosphine (TCEP), a sulfur-free reducing agent, it was possible to obtain well ordered crystals of wild-type hAOX1 and a variant, hAOX1_6A, which diffracted beyond 2.3\u2005\u00c5 resolution. Instead of the typical star-shaped crystals of hAOX1, at pH 4.7 plates are obtained in the orthorhombic space group\n                    <jats:italic>P<\/jats:italic>\n                    2\n                    <jats:sub>1<\/jats:sub>\n                    2\n                    <jats:sub>1<\/jats:sub>\n                    2 with two molecules in the asymmetric unit. Activity assays with the enzyme incubated with both reducing agents show that in contrast to DTT, TCEP did not inactivate hAOX1. The replacement of DTT with TCEP in the crystallization of hAOX1 provides a strategy to circumvent enzyme inactivation during crystallographic studies, allowing future applications of new assays, such as time-resolved crystallography.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26006904","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:18:02Z","timestamp":1784711882000},"page":"275-282","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Structure of human aldehyde oxidase under tris(2-carboxyethyl)phosphine reducing conditions"],"prefix":"10.1107","volume":"82","author":[{"given":"Carolina","family":"Videira","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["UCIBIO, Applied Molecular Biosciences Unit and Associate Laboratory i4HB \u2013 Institute for Health and Bioeconomy, Department of Chemistry NOVA School of Science and Technology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Esmaeeli","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bnmw459","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Enzymology, Institute of Biochemistry and Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Leimk\u00fchler","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03bnmw459","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Molecular Enzymology, Institute of Biochemistry and Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jo\u00e3o","family":"Rom\u00e3o","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["UCIBIO, Applied Molecular Biosciences Unit and Associate Laboratory i4HB \u2013 Institute for Health and Bioeconomy, Department of Chemistry NOVA School of Science and Technology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiano","family":"Mota","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xankh89","id-type":"ROR","asserted-by":"publisher"}],"department":["UCIBIO, Applied Molecular Biosciences Unit and Associate Laboratory i4HB \u2013 Institute for Health and Bioeconomy, Department of Chemistry NOVA School of Science and Technology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"no5217_bb1","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/S0006-291X(02)00303-0","volume":"293","author":"Buechler","year":"2002","journal-title":"Biochem. 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