{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T11:10:50Z","timestamp":1791025850716,"version":"4.1.0"},"reference-count":26,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2026,7,20]]},"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>\n                    L-Galactose dehydrogenase plays a key role in the biosynthesis of L-ascorbic acid (vitamin C) in plants. Here, we report crystal structures of rice (\n                    <jats:italic>Oryza sativa<\/jats:italic>\n                    ) L-galactose dehydrogenase in both apo and NAD\n                    <jats:sup>+<\/jats:sup>\n                    -bound forms at 1.2 and 1.8\u2005\u00c5 resolution, respectively. Rice L-galactose dehydrogenase adopts a conserved (\u03b2\/\u03b1)\n                    <jats:sub>8<\/jats:sub>\n                    -barrel fold with a well preserved NAD\n                    <jats:sup>+<\/jats:sup>\n                    -binding cleft, as in other plant L-galactose dehydrogenases. Structural comparisons revealed conformational flexibility in the first and second loop regions, which form the lid and side wall of the NAD\n                    <jats:sup>+<\/jats:sup>\n                    -binding site. These loops undergo significant rearrangement upon NAD\n                    <jats:sup>+<\/jats:sup>\n                    binding, likely facilitating cofactor and substrate uptake. These findings suggest a flexible loop-mediated regulation of cofactor and substrate access.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26007430","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T09:38:13Z","timestamp":1785231493000},"page":"289-294","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":1,"title":["Crystal structure of rice\n                    <scp>L<\/scp>\n                    -galactose dehydrogenase"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3551-6854","authenticated-orcid":true,"given":"Zui","family":"Fujimoto","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023v4bd62","id-type":"ROR","asserted-by":"publisher"}],"department":["Research Center for Advanced Analysis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5417-1728","authenticated-orcid":true,"given":"Mitsuru","family":"Momma","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023v4bd62","id-type":"ROR","asserted-by":"publisher"}],"department":["Research Center for Advanced Analysis"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8420-5852","authenticated-orcid":true,"given":"Nobuhiro","family":"Suzuki","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023v4bd62","id-type":"ROR","asserted-by":"publisher"}],"department":["Research Center for Advanced Analysis"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"nw5139_bb99","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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