{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:33:54Z","timestamp":1787924034593,"version":"build-2784847793"},"reference-count":26,"publisher":"International Union of Crystallography (IUCr)","issue":"9","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.iucr.org\/services\/copyrightpolicy.html#TDM"}],"funder":[{"DOI":"10.13039\/100010858","name":"Texas A&M University-Commerce","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2026,7,20]]},"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>NKX2-1 (thyroid transcription factor 1, TTF-1) is a homeodomain transcription factor that plays critical roles in the development and function of the thyroid, lung and forebrain. Here, we report the crystal structure of the NKX2-1 homeodomain bound to a 19\u2005bp DNA duplex containing two palindromically arranged NK2-recognition motifs, refined to 3.26\u2005\u00c5 resolution. The structure reveals two homeodomains bound to a single DNA duplex and demonstrates that the overall fold and DNA-binding interactions are highly conserved relative to those of NKX2-5. Comparison with NKX2-5 further shows that the amino-acid residues that differ between the two homeodomains are located away from the protein\u2013DNA interface, suggesting that functional differences between these transcription factors are unlikely to arise from distinct DNA-recognition mechanisms. These findings provide a structural framework for understanding DNA recognition by NKX2-1 and for interpreting the effects of pathogenic variants within its homeodomain.<\/jats:p>","DOI":"10.1107\/s2053230x26007466","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T09:11:01Z","timestamp":1785921061000},"page":"295-300","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Crystal structure of the NKX2-1 homeodomain bound to a palindromic DNA recognition sequence"],"prefix":"10.1107","volume":"82","author":[{"given":"Aiswarya","family":"Mohandas","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01red3556","id-type":"ROR","asserted-by":"publisher"}],"department":["Biological and Environmental Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0235-9311","authenticated-orcid":true,"given":"Hyun-Joo","family":"Nam","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01red3556","id-type":"ROR","asserted-by":"publisher"}],"department":["Biological and Environmental Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"ek5043_bb1","doi-asserted-by":"crossref","first-page":"E4","DOI":"10.1038\/s41586-024-08416-7","volume":"636","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"ek5043_bb2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Cryst. 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