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These complexes include various lanthanide and main-group metal(III) ions whose radioisotopes are useful in nuclear medicine, with ionic radii ranging from 0.62 to 1.03\u2005\u00c5. Binding activities of the Anticalin for the\n                    <jats:italic>M<\/jats:italic>\n                    <jats:sup>III<\/jats:sup>\n                    \u00b7DTPA-NH\n                    <jats:sub>2<\/jats:sub>\n                    complexes were quantified by fluorescence titration (probing the intrinsic Tyr\/Trp emission), revealing\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    values of 0.8\u20132.4\u2005n\n                    <jats:italic>M<\/jats:italic>\n                    for most of the lanthanide ions investigated (\n                    <jats:italic>r<\/jats:italic>\n                    <jats:sub>ion<\/jats:sub>\n                    = 0.75\u20130.96\u2005\u00c5), but showing markedly reduced affinities towards the small and large main-group metal ions Ga\n                    <jats:sup>3+<\/jats:sup>\n                    (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    = 15.3\u2005nM) and Bi\n                    <jats:sup>3+<\/jats:sup>\n                    (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    = 47.8\u2005n\n                    <jats:italic>M<\/jats:italic>\n                    ), respectively. The crystal structures of six representative\n                    <jats:italic>M<\/jats:italic>\n                    <jats:sup>III<\/jats:sup>\n                    \u00b7DTPA-NH\n                    <jats:sub>2<\/jats:sub>\n                    complexes bound to the Anticalin were solved at high resolution (1.5\u20131.8\u2005\u00c5) using synchrotron X-ray diffraction. Superposition onto the previously described Anticalin CL31 with bound Y\n                    <jats:sup>3+<\/jats:sup>\n                    \u00b7DTPA-SCN indicated an essentially invariant conformation both for the binding protein and its metal-chelate ligands, including conserved hydrogen bonds, and a surprisingly uniform ninefold metal coordination via five carboxylate groups, three N atoms and one water molecule. However, there were two exceptions: the small Sc\n                    <jats:sup>3+<\/jats:sup>\n                    ion appeared to be coordinated only eightfold with the DTPA-NH\n                    <jats:sub>2<\/jats:sub>\n                    chelator but lacking the water ligand, whereas no metal electron density was observed for the Ga\n                    <jats:sup>3+<\/jats:sup>\n                    ion, in line with its known noncanonical DTPA complex geometry. Interestingly, in this case a water molecule was detected at the expected position of the central metal ion within the protein-bound DTPA-NH\n                    <jats:sub>2<\/jats:sub>\n                    chelator. Our investigation of the influence of chelate geometry on complex stability establishes the Anticalin CL31d as a small and robust universal binding protein for medically relevant\n                    <jats:italic>M<\/jats:italic>\n                    <jats:sup>III<\/jats:sup>\n                    \u00b7DTPA complexes with surprisingly broad tolerance towards varying ionic radii, thus enabling flexible radionuclide-targeting strategies in nuclear medicine.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26007624","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:42:53Z","timestamp":1786365773000},"page":"301-311","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Diverse DTPA-chelated lanthanides with relevance for nuclear medicine bound to an engineered lipocalin reveal conserved ligand geometry"],"prefix":"10.1107","volume":"82","author":[{"given":"Alina","family":"Mirwald","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kkvpp62","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Eichinger","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kkvpp62","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5717-498X","authenticated-orcid":true,"given":"Arne","family":"Skerra","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kkvpp62","id-type":"ROR","asserted-by":"publisher"}],"department":["School of Life Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"rl5209_bb1","first-page":"100054","volume":"6","author":"Achatz","year":"2022","journal-title":"J. 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