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Here, we present the crystal structure of Pth2 from\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Lokiarchaeum sp. GC14_75 (\n                    <jats:italic>Loki<\/jats:italic>\n                    Pth2) at 2.12\u2005\u00c5 resolution. This is the first structure of Pth2 from a lineage within Promethearchaeati, a kingdom of archaea closely related to eukaryotes. The structure reveals that\n                    <jats:italic>Loki<\/jats:italic>\n                    Pth2 forms a homodimer and closely resembles Pth2 structures from other species. However,\n                    <jats:italic>Loki<\/jats:italic>\n                    Pth2 exhibits two prominent structural differences: a short helix around the catalytic center, which is absent in other Pth2s, and a distinct orientation of the C-terminal helix. Detailed comparative structural analysis suggests that these regions may regulate enzymatic activity and substrate binding, respectively. Furthermore, the corresponding regions in other Pth2s also exhibit high flexibility, suggesting that similar mechanisms may be conserved among Pth2s. To gain insights into the growth environment of\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Lokiarchaeum sp. GC14_75, we assess the optimal temperature for the catalytic reaction of\n                    <jats:italic>Loki<\/jats:italic>\n                    Pth2, which suggests that\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Lokiarchaeum sp. GC14_75 inhabits moderately thermophilic environments.\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26008423","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T11:46:43Z","timestamp":1787140003000},"page":"320-328","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Structural and functional analysis of peptidyl-tRNA hydrolase 2 from\n                    <i>Candidatus<\/i>\n                    Lokiarchaeum sp. GC14_75"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3425-5543","authenticated-orcid":true,"given":"Aoi","family":"Kawashima","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04ww21r56","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Life and Food Sciences, Graduate School of Science and Technology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5161-1301","authenticated-orcid":true,"given":"Kosuke","family":"Ito","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04ww21r56","id-type":"ROR","asserted-by":"publisher"}],"department":["Department of Life and Food Sciences, Graduate School of Science and Technology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"us5165_bb2","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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