{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T11:38:29Z","timestamp":1794397109579,"version":"4.9.0"},"reference-count":26,"publisher":"International Union of Crystallography (IUCr)","issue":"10","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr F Struct Biol Commun","Acta Crystallogr F","Acta Crystallogr F Struct Biol Cryst Commun","Acta Cryst F","Acta Cryst F Struct Biol Commun","Acta Cryst Sect F","Acta Cryst Sect F Struct Biol Commun","Acta Crystallogr Sect F","Acta Crystallogr Sect F Struct Biol Commun","Acta Crystallogr Sect F Struct Biol Cryst Commun"],"accepted":{"date-parts":[[2026,9,1]]},"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>\n                    The thermostable GH10 xylanase from\n                    <jats:italic>Thermoascus aurantiacus<\/jats:italic>\n                    (TaXyn10) is an industrially relevant enzyme whose native structure contains an N-terminal pyroglutamate (PCA) modification. Recombinant production of proteins containing an N-terminal PCA is often hindered by purification strategies that disrupt the authentic N-terminus. Here, we report the 1.1\u2005\u00c5 resolution crystal structure of TaXyn10 recombinantly produced in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and purified using a split-intein-based system. The structure closely resembles that of the native protein. Electron-density maps support the presence of an intact N-terminal PCA, with partial occupancy consistent with spontaneous cyclization of the exposed N-terminal glutamine after intein cleavage. These results demonstrate that split-intein-based purification enables recovery of the native mature state of TaXyn10 and can preserve physiologically relevant N-terminal PCA formation in recombinant protein produced in\n                    <jats:italic>E. coli<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1107\/s2053230x26009155","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:15:50Z","timestamp":1789564550000},"page":"329-335","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":0,"title":["Structure of recombinant\n                    <i>Thermoascus aurantiacus<\/i>\n                    xylanase TaXyn10 with an authentic N-terminal pyroglutamate"],"prefix":"10.1107","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8353-8794","authenticated-orcid":true,"given":"Jelena","family":"Mijatovic","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02aj13c28","id-type":"ROR","asserted-by":"publisher"}],"department":["Macromolecular Crystallography"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7139-0718","authenticated-orcid":true,"given":"Uwe","family":"Mueller","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02aj13c28","id-type":"ROR","asserted-by":"publisher"}],"department":["Macromolecular Crystallography"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-7047","authenticated-orcid":true,"given":"Manfred S.","family":"Weiss","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02aj13c28","id-type":"ROR","asserted-by":"publisher"}],"department":["Macromolecular Crystallography"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Lennartz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02aj13c28","id-type":"ROR","asserted-by":"publisher"}],"department":["Macromolecular Crystallography"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"ada5002_bb23","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1107\/S2059798323003595","volume":"79","author":"Agirre","year":"2023","journal-title":"Acta Cryst. 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