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Crystal structures were determined of the N-terminal domain (NTD) from crystals grown at pH 8.0 to a resolution of 1.94\u2005\u00c5 and of the C-terminal domain (CTD) from crystals grown at pH 5.0 and 8.5 to resolutions of 1.59 and 1.30\u2005\u00c5, respectively. The NTD adopts the canonical coronavirus NTD fold and contains a positively charged cleft corresponding to a previously proposed RNA-binding region. Comparison of the apo NTD structure determined in this study with a previously reported RNA-bound NTD structure revealed local differences in the side-chain conformations of RNA-contacting residues, including Thr49, Arg88, Arg92, Arg107, Tyr109 and Tyr111, whereas the overall domain fold remained essentially unchanged. These observations suggest that RNA binding may be accommodated by local side-chain rearrangements within a pre-existing positively charged cleft rather than by a large-scale conformational change. 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