{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:46:02Z","timestamp":1792230362677,"version":"4.4.0"},"reference-count":27,"publisher":"International Union of Crystallography (IUCr)","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/uk\/legalcode"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/uk\/legalcode"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["WT106121MA"],"award-info":[{"award-number":["WT106121MA"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iucr.org","wiley.com","iucrj.org"],"crossmark-restriction":true},"short-container-title":["Acta Crystallogr D Struct Biol","Acta Crystallogr D","Acta Cryst D","Acta Cryst D Struct Biol","Acta Cryst Sect D","Acta Cryst Sect D Struct Biol","Acta Crystallogr Sect D","Acta Crystallogr Sect D Struct Biol","Acta Crystallogr D Biol Crystallogr","Acta Cryst D Biol Cryst"],"published-print":{"date-parts":[[2018,1,1]]},"abstract":"<jats:p>\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    causes a severe disease that kills thousands of people annually. The outer membrane protein OmpU is the most abundant outer membrane protein in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    , and has been identified as an important virulence factor that is involved in host-cell interaction and recognition, as well as being critical for the survival of the pathogenic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    in the host body and in harsh environments. The mechanism of these processes is not well understood owing to a lack of the structure of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    OmpU. Here, the crystal structure of the\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    OmpU trimer is reported to a resolution of 2.2\u2005\u00c5. The protomer forms a 16-\u03b2-stranded barrel with a noncanonical N-terminal coil located in the lumen of the barrel that consists of residues Gly32\u2013Ser42 and is observed to participate in forming the second gate in the pore. By mapping the published functional data onto the OmpU structure, the OmpU structure reinforces the notion that the long extracellular loop L4 with a \u03b2-hairpin-like motif may be critical for host-cell binding and invasion, while L3, L4 and L8 are crucially implicated in phage recognition by\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1107\/s2059798317017697","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T10:48:59Z","timestamp":1513939739000},"page":"21-29","update-policy":"https:\/\/doi.org\/10.1107\/cm_01","source":"Crossref","is-referenced-by-count":25,"title":["Crystal structure of the outer membrane protein OmpU from\n                    <i>Vibrio cholerae<\/i>\n                    at 2.2\u2005\u00c5 resolution"],"prefix":"10.1107","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5482-9936","authenticated-orcid":true,"given":"Huanyu","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjiang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"329","published-online":{"date-parts":[[2018,1,1]]},"reference":[{"key":"jb5001_bb35","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.jmb.2006.08.002","volume":"362","author":"Basl\u00e9","year":"2006","journal-title":"J. Mol. Biol."},{"key":"jb5001_bb2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1107\/S0907444910048675","volume":"67","author":"Battye","year":"2011","journal-title":"Acta Cryst. D"},{"key":"jb5001_bb3","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1111\/jfd.12036","volume":"36","author":"Cai","year":"2013","journal-title":"J. Fish Dis."},{"key":"jb5001_bb4","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1128\/jb.178.2.524-530.1996","volume":"178","author":"Chakrabarti","year":"1996","journal-title":"J. Bacteriol."},{"key":"jb5001_bb5","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1073\/pnas.1015326108","volume":"108","author":"Duperthuy","year":"2011","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"jb5001_bb6","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.1016\/j.bpj.2010.01.010","volume":"98","author":"Duret","year":"2010","journal-title":"Biophys. J."},{"key":"jb5001_bb32","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0969-2126(99)80055-0","volume":"7","author":"Dutzler","year":"1999","journal-title":"Structure Fold. Des."},{"key":"jb5001_bb8","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Cryst. D"},{"key":"jb5001_bb9","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1107\/S0907444905036693","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Cryst. D"},{"key":"jb5001_bb10","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1107\/S090744491003982X","volume":"67","author":"Evans","year":"2011","journal-title":"Acta Cryst. D"},{"key":"jb5001_bb12","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","volume":"10","author":"Kelley","year":"2015","journal-title":"Nature Protoc."},{"key":"jb5001_bb13","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.pep.2012.08.005","volume":"85","author":"Khan","year":"2012","journal-title":"Protein Expr. Purif."},{"key":"jb5001_bb14","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1107\/S0907444992008886","volume":"49","author":"Lamzin","year":"1993","journal-title":"Acta Cryst. D"},{"key":"jb5001_bb15","doi-asserted-by":"crossref","first-page":"e0119026","DOI":"10.1371\/journal.pone.0119026","volume":"10","author":"Liu","year":"2015","journal-title":"PLoS One"},{"key":"jb5001_bb16","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1128\/IAI.72.6.3577-3583.2004","volume":"72","author":"Mathur","year":"2004","journal-title":"Infect. Immun."},{"key":"jb5001_bb17","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1128\/jb.170.6.2575-2583.1988","volume":"170","author":"Miller","year":"1988","journal-title":"J. Bacteriol."},{"key":"jb5001_bb18","doi-asserted-by":"crossref","first-page":"10220","DOI":"10.1073\/pnas.170219997","volume":"97","author":"Provenzano","year":"2000","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"jb5001_bb19","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1128\/IAI.68.3.1491-1497.2000","volume":"68","author":"Provenzano","year":"2000","journal-title":"Infect. Immun."},{"key":"jb5001_bb20","doi-asserted-by":"crossref","first-page":"W320","DOI":"10.1093\/nar\/gku316","volume":"42","author":"Robert","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"jb5001_bb21","doi-asserted-by":"crossref","first-page":"e03497","DOI":"10.7554\/eLife.03497","volume":"3","author":"Seed","year":"2014","journal-title":"Elife"},{"key":"jb5001_bb23","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/S0263-7855(97)00009-X","volume":"14","author":"Smart","year":"1996","journal-title":"J. Mol. Graph."},{"key":"jb5001_bb24","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1128\/IAI.63.11.4433-4438.1995","volume":"63","author":"Sperandio","year":"1995","journal-title":"Infect. Immun."},{"key":"jb5001_bb25","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1107\/S0907444904023510","volume":"60","author":"Vagin","year":"2004","journal-title":"Acta Cryst. D"},{"key":"jb5001_bb27","doi-asserted-by":"crossref","first-page":"599","DOI":"10.3390\/ijms17040599","volume":"17","author":"Vollan","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"jb5001_bb28","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1128\/IAI.70.1.121-126.2002","volume":"70","author":"Wibbenmeyer","year":"2002","journal-title":"Infect. Immun."},{"key":"jb5001_bb30","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Cryst. D"},{"key":"jb5001_bb31","unstructured":"World Health Organization (2012). Cholera Fact Sheet. Geneva: World Health Organization. http:\/\/www.who.int\/mediacentre\/factsheets\/fs107\/en\/."}],"container-title":["Acta Crystallographica Section D Structural Biology"],"original-title":[],"link":[{"URL":"http:\/\/journals.iucr.org\/d\/issues\/2018\/01\/00\/jb5001\/jb5001.pdf","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.iucr.org\/d\/issues\/2018\/01\/00\/jb5001\/jb5001.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T05:48:18Z","timestamp":1736747298000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.iucr.org\/paper?S2059798317017697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,1]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["S2059798317017697"],"URL":"https:\/\/doi.org\/10.1107\/s2059798317017697","relation":{},"ISSN":["2059-7983"],"issn-type":[{"value":"2059-7983","type":"print"}],"subject":[],"published":{"date-parts":[[2018,1,1]]},"assertion":[{"value":"Acta Crystallographica Section D: Structural Biology","order":0,"name":"journal","label":"Publication","explanation":{"URL":"http:\/\/journals.iucr.org\/d"},"group":{"name":"general","label":"General"}},{"value":"research papers","order":1,"name":"content_type","label":"Content type","explanation":{"URL":"https:\/\/journals.iucr.org\/d\/services\/notesforauthors.html"},"group":{"name":"general","label":"General"}},{"value":"Available","URL":"http:\/\/scripts.iucr.org\/cgi-bin\/citedin?jb5001","order":2,"name":"article_metrics","label":"Article metrics","group":{"name":"general","label":"General"}},{"value":"Yes","order":0,"name":"peer_reviewed","label":"Peer reviewed","group":{"name":"peer_review","label":"Peer review"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review process","group":{"name":"peer_review","label":"Peer review"}},{"value":"26 October 2017","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication history"}},{"value":"12 December 2017","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication history"}},{"value":"1 January 2018","order":2,"name":"published_online","label":"Published online","group":{"name":"publication_history","label":"Publication history"}},{"value":"This article has supporting information","name":"supplementary_materials","label":"Supplementary materials","explanation":{"URL":"http:\/\/scripts.iucr.org\/cgi-bin\/sendsup?jb5001"}},{"value":"\u00a9 2018 Li\n                      et al.","order":0,"name":"copyright","label":"Copyright","explanation":{"URL":"http:\/\/journals.iucr.org\/services\/copyrightpolicy.html"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}},{"value":"Creative Commons Attribution (CC-BY)","order":1,"name":"license","label":"Licence","explanation":{"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/uk\/legalcode"},"group":{"name":"copyright_and_licencing","label":"Copyright and licencing"}}]}}